1H NMR-based metabolite profiling of diet-induced obesity in a mouse mode
High-fat diets (HFD) and high-carbohydrate diets (HCD)- induced obesity through different pathways, but the metabolic differences between these diets are not fully understood. Therefore, we applied proton nuclear magnetic resonance ((1)H NMR)-based metabolomics to compare the metabolic patterns betw...
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Published in | BMB reports Vol. 45; no. 7; pp. 419 - 424 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
Korean Society for Biochemistry and Molecular Biology
01.07.2012
생화학분자생물학회 |
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Abstract | High-fat diets (HFD) and high-carbohydrate diets (HCD)- induced obesity through different pathways, but the metabolic differences between these diets are not fully understood. Therefore, we applied proton nuclear magnetic resonance ((1)H NMR)-based metabolomics to compare the metabolic patterns between C57BL/6 mice fed HCD and those fed HFD. Principal component analysis derived from (1)H NMR spectra of urine showed a clear separation between the HCD and HFD groups. Based on the changes in urinary metabolites, the slow rate of weight gain in mice fed the HCD related to activation of the tricarboxylic acid cycle (resulting in increased levels of citrate and succinate in HCD mice), while the HFD affected nicotinamide metabolism (increased levels of 1-methylnicotineamide, nicotinamide-N-oxide in HFD mice), which leads to systemic oxidative stress. In addition, perturbation of gut microflora metabolism was also related to different metabolic patterns of those two diets. These findings demonstrate that (1)H NMR-based metabolomics can identify diet-dependent perturbations in biological pathways. |
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AbstractList | High-fat diets (HFD) and high-carbohydrate diets (HCD)-induced obesity through different pathways, but the metabolic differences between these diets are not fully understood.
Therefore, we applied proton nuclear magnetic resonance (1H NMR)-based metabolomics to compare the metabolic patterns between C57BL/6 mice fed HCD and those fed HFD. Principal component analysis derived from 1H NMR spectra of urine showed a clear separation between the HCD and HFD groups.
Based on the changes in urinary metabolites, the slow rate of weight gain in mice fed the HCD related to activation of the tricarboxylic acid cycle (resulting in increased levels of citrate and succinate in HCD mice), while the HFD affected nicotinamide metabolism (increased levels of 1-methylnicotineamide,nicotinamide-N-oxide in HFD mice), which leads to systemic oxidative stress. In addition, perturbation of gut microflora metabolism was also related to different metabolic patterns of those two diets. These findings demonstrate that 1H NMRbased metabolomics can identify diet-dependent perturbations in biological pathways. [BMB Reports 2012; 45(7):419-424] KCI Citation Count: 27 High-fat diets (HFD) and high-carbohydrate diets (HCD)-induced obesity through different pathways, but the metabolicdifferences between these diets are not fully understood.Therefore, we applied proton nuclear magnetic resonance (1HNMR)-based metabolomics to compare the metabolic patternsbetween C57BL/6 mice fed HCD and those fed HFD. Principalcomponent analysis derived from 1H NMR spectra of urineshowed a clear separation between the HCD and HFD groups.Based on the changes in urinary metabolites, the slow rate ofweight gain in mice fed the HCD related to activation of thetricarboxylic acid cycle (resulting in increased levels of citrateand succinate in HCD mice), while the HFD affected nicotinamidemetabolism (increased levels of 1-methylnicotineamide,nicotinamide-N-oxide in HFD mice), which leads to systemicoxidative stress. In addition, perturbation of gut microflorametabolism was also related to different metabolic patterns ofthose two diets. These findings demonstrate that 1H NMRbasedmetabolomics can identify diet-dependent perturbationsin biological pathways. High-fat diets (HFD) and high-carbohydrate diets (HCD)- induced obesity through different pathways, but the metabolic differences between these diets are not fully understood. Therefore, we applied proton nuclear magnetic resonance ((1)H NMR)-based metabolomics to compare the metabolic patterns between C57BL/6 mice fed HCD and those fed HFD. Principal component analysis derived from (1)H NMR spectra of urine showed a clear separation between the HCD and HFD groups. Based on the changes in urinary metabolites, the slow rate of weight gain in mice fed the HCD related to activation of the tricarboxylic acid cycle (resulting in increased levels of citrate and succinate in HCD mice), while the HFD affected nicotinamide metabolism (increased levels of 1-methylnicotineamide, nicotinamide-N-oxide in HFD mice), which leads to systemic oxidative stress. In addition, perturbation of gut microflora metabolism was also related to different metabolic patterns of those two diets. These findings demonstrate that (1)H NMR-based metabolomics can identify diet-dependent perturbations in biological pathways. |
Author | Shin, Jae Hoon Lee, Ho-Sub Kim, Yo Na Jung, Jee-youn Jang, Zi-hey Kim, Jin-sup Kim, Il Yong Hwang, Geum-Sook Seong, Je Kyung |
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SubjectTerms | 1H NMR Animals Diet Disease Models, Animal Etabolomics High-carbohydrate diet High-fat diet Magnetic Resonance Spectroscopy - methods Male Metabolite profiling Mice Mice, Inbred C57BL Obesity - etiology Obesity - metabolism Protons 화학 |
Title | 1H NMR-based metabolite profiling of diet-induced obesity in a mouse mode |
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