The Effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) Inhibition on Metabolic Flexibility during Endurance Training in Skeletal Muscles of Streptozotocin-induced Diabetic Rats
Background:Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. Pyruvate Dehydrogenase Kinase 4 (PDK4) is one of the main enzymes that play a critical role in metabolic flexibility. In current study, we examine...
Saved in:
Published in | Journal of Kerman University of Medical Sciences Vol. 27; no. 4; pp. 304 - 317 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Kerman University of Medical Sciences
01.07.2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Background:Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. Pyruvate Dehydrogenase Kinase 4 (PDK4) is one of the main enzymes that play a critical role in metabolic flexibility. In current study, we examined PDK4 inhibition along with exercise training (ET) on the gene expression of Estrogen related-receptor alpha (ERRα), medium-chain acyl-CoA dehydrogenase (MCAD), carnitine palmitoyl transferase-1b (CPT-1b), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), PDK4 and citrate synthase (CS) in skeletal muscle.Method:Sixty-four male Wistar rats (8 week-old) were randomly divided into 8 groups (n=8); 1- untreated control, 2- STZ-induced diabetic, 3- PDK4 inhibition, 4- endurance training (ET), 5- diabetic + PDK4 inhibition, 6- diabetic + ET, 7- PDK4 inhibition + ET, and 8- diabetic +ET + PDK4 inhibition. ERRα, MCAD, CPT-1b, PGC-1α, PDK4 and CS genes expressions were measured by Real-Time PCR and quantified by 2-ΔΔCt method.Results:ERRα, MCAD, CPT-1b, PGC-1α, PDK4, and CS expressions were significantly higher in non-diabetic+ Endurance Training group compared to the control group. The expressions of CPT-1b, MCAD and CS genes were significantly lower in the non-diabetic+ endurance training/PDK4 inhibition compared to the non-diabetic+ endurance training group, and the expressions of ERRα, CPT-1b and MCAD were significantly lower in the diabetic + PDK4 inhibition group compared to the diabetic group.Conclusion:In sum, PDK4 inhibition has negative effects on lipid metabolism in healthy rats, but in animals with diabetes, PDK4 inhibition can be used for improving lipid metabolism (over-expression of CS and PGC-1α). |
---|---|
AbstractList | Background:Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. Pyruvate Dehydrogenase Kinase 4 (PDK4) is one of the main enzymes that play a critical role in metabolic flexibility. In current study, we examined PDK4 inhibition along with exercise training (ET) on the gene expression of Estrogen related-receptor alpha (ERRα), medium-chain acyl-CoA dehydrogenase (MCAD), carnitine palmitoyl transferase-1b (CPT-1b), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), PDK4 and citrate synthase (CS) in skeletal muscle.Method:Sixty-four male Wistar rats (8 week-old) were randomly divided into 8 groups (n=8); 1- untreated control, 2- STZ-induced diabetic, 3- PDK4 inhibition, 4- endurance training (ET), 5- diabetic + PDK4 inhibition, 6- diabetic + ET, 7- PDK4 inhibition + ET, and 8- diabetic +ET + PDK4 inhibition. ERRα, MCAD, CPT-1b, PGC-1α, PDK4 and CS genes expressions were measured by Real-Time PCR and quantified by 2-ΔΔCt method.Results:ERRα, MCAD, CPT-1b, PGC-1α, PDK4, and CS expressions were significantly higher in non-diabetic+ Endurance Training group compared to the control group. The expressions of CPT-1b, MCAD and CS genes were significantly lower in the non-diabetic+ endurance training/PDK4 inhibition compared to the non-diabetic+ endurance training group, and the expressions of ERRα, CPT-1b and MCAD were significantly lower in the diabetic + PDK4 inhibition group compared to the diabetic group.Conclusion:In sum, PDK4 inhibition has negative effects on lipid metabolism in healthy rats, but in animals with diabetes, PDK4 inhibition can be used for improving lipid metabolism (over-expression of CS and PGC-1α). |
Author | Beydolah Shahouzehi Saeed Shakerian Soheil Aminizadeh Hamid Marefati Yaser Masoumi-Ardakani Abdolhamid Habibi |
Author_xml | – sequence: 1 fullname: Hamid Marefati organization: Associate Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran – sequence: 2 fullname: Yaser Masoumi-Ardakani organization: Ph.D. Candidate, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran – sequence: 3 fullname: Saeed Shakerian organization: Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran – sequence: 4 fullname: Abdolhamid Habibi organization: Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran – sequence: 5 fullname: Soheil Aminizadeh organization: Assistant Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran – sequence: 6 fullname: Beydolah Shahouzehi organization: Assistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences & Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran |
BookMark | eNotT8tOwzAQjBAcoHDn6CMcUhzbcZIjoi1UpQJB75Ef69ZtsJHjIsJP8YuYgrTSrGZ3ZnbPsmPnHWTZZYHHhGBObra7t_2YYILHTYGL6ig7JRjXOakZPc2-VxtAU2NAxR55g56HsP8QEdAENoMOfg1O9IAW9gAMXT1PFuwazd3GShutdyjVEqKQvrMKzTr4TIPOxgHpfbBujaYuNcIpQKsgrPulrEOvO-iSqkPLfa86OGS_xgDv0X_56JV1uU1CBRpNrJAQk_mLiP15dmJE18PFP46y1Wy6unvIH5_u53e3j7kuKI95aYTEmBeFYVRKLX-fTVBVmOkaZFEzkJWSlPO0CE1Jm5orMLoBJUtM6Cib_9lqL7bte7BvIgytF7Y9ED6sWxHSTR20hjBesaos61IyTrgUDcWY6qapdcWwoT-kgH3r |
ContentType | Journal Article |
DBID | DOA |
DOI | 10.22062/jkmu.2020.91017 |
DatabaseName | DOAJ Directory of Open Access Journals |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
DeliveryMethod | fulltext_linktorsrc |
EISSN | 2008-2843 |
EndPage | 317 |
ExternalDocumentID | oai_doaj_org_article_f2467475585b4626ba93003d998d740f |
GroupedDBID | GROUPED_DOAJ |
ID | FETCH-LOGICAL-d136t-5fab00611f43bbdb2843bdb7704d8eb184eb7cb366fabe953986cefd9ecb5023 |
IEDL.DBID | DOA |
IngestDate | Tue Oct 22 15:01:24 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-d136t-5fab00611f43bbdb2843bdb7704d8eb184eb7cb366fabe953986cefd9ecb5023 |
OpenAccessLink | https://doaj.org/article/f2467475585b4626ba93003d998d740f |
PageCount | 14 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_f2467475585b4626ba93003d998d740f |
PublicationCentury | 2000 |
PublicationDate | 2020-07-01 |
PublicationDateYYYYMMDD | 2020-07-01 |
PublicationDate_xml | – month: 07 year: 2020 text: 2020-07-01 day: 01 |
PublicationDecade | 2020 |
PublicationTitle | Journal of Kerman University of Medical Sciences |
PublicationYear | 2020 |
Publisher | Kerman University of Medical Sciences |
Publisher_xml | – name: Kerman University of Medical Sciences |
Score | 2.186508 |
Snippet | Background:Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability.... |
SourceID | doaj |
SourceType | Open Website |
StartPage | 304 |
SubjectTerms | pyruvate dehydrogenase kinase 4 endurance training metabolic flexibility estrogen related receptor |
Title | The Effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) Inhibition on Metabolic Flexibility during Endurance Training in Skeletal Muscles of Streptozotocin-induced Diabetic Rats |
URI | https://doaj.org/article/f2467475585b4626ba93003d998d740f |
Volume | 27 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3PS8MwFA7iyYsoKv7mHTzooa4_0rQ9qtuYjsnQCbuVpElYnbZja4X5T_kv-tL0oCcvQiFtKQm8L837HvnyHiEXgssIsaVOTHXk0IBxJ_YCDFwpF5IlScZFI5B9ZIMX-jANpz9KfRlNmE0PbA3X0b6phxGFSGsFRfYteBLgTJQYJsiIurpZfb3QBlN2H9L3XeZ3XufvNcZ_vnudmGn3Kyd_4zz6O2S7ZX1wY0fbJRuq2CNfCBHY7MErKDWM18v6A6kfdNVsLZclQosuBoZ501C4HHeH9Arui1kuGqEV4DVSFcL4lmfQN6ktG6nrGuzhQ-gVeGNghUlbCQLyAp7n6GqQc8OoXhlNnBnbbE4vqvKzrBCswsFAHSGXYPUy2PkTr1b7ZNLvTe4GTls_wZFo7soJNTc_ledpGgghBXqiAJsocqmMcY2OqRJRJgLG8EOVhEESs0xpmahMhOjLD8hmURbqkIDGZ81DHWvOKedRwnzKA6U09TTDgPCI3BrjpgubISM1OaubF4hk2iKZ_oXk8X90ckK2DOZWUHtKNqtlrc6QNlTivJkh33NNxLY |
link.rule.ids | 315,786,790,870,2115,27955,27956 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+Effects+of+Pyruvate+Dehydrogenase+Kinase+4+%28PDK4%29+Inhibition+on+Metabolic+Flexibility+during+Endurance+Training+in+Skeletal+Muscles+of+Streptozotocin-induced+Diabetic+Rats&rft.jtitle=Journal+of+Kerman+University+of+Medical+Sciences&rft.au=Hamid+Marefati&rft.au=Yaser+Masoumi-Ardakani&rft.au=Saeed+Shakerian&rft.au=Abdolhamid+Habibi&rft.date=2020-07-01&rft.pub=Kerman+University+of+Medical+Sciences&rft.eissn=2008-2843&rft.volume=27&rft.issue=4&rft.spage=304&rft.epage=317&rft_id=info:doi/10.22062%2Fjkmu.2020.91017&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_f2467475585b4626ba93003d998d740f |