DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii Vol. 92; no. 6; pp. 82 - 86
• Main Authors: Petrov, A A, Pyshnaya, N S, Lebedev, V N, Kulish, V S, Stovba, L F, Kazantsev, A V, Borisevich, S V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) Central Research Institute for Epidemiology 01.11.2015
• Subjects: Alphavirus Infections - diagnosis; Alphavirus Infections - virology; Animals; Animals, Outbred Strains; biological sample; Chickens; Encephalitis Virus, Japanese - genetics; Encephalitis Virus, Japanese - isolation & purification; Encephalitis Virus, Venezuelan Equine - genetics; Encephalitis Virus, Venezuelan Equine - isolation & purification; Encephalitis Viruses, Tick-Borne - genetics; Encephalitis Viruses, Tick-Borne - isolation & purification; Encephalitis, Japanese - diagnosis; Encephalitis, Japanese - virology; Encephalitis, Tick-Borne - diagnosis; Encephalitis, Tick-Borne - virology; Encephalomyelitis, Venezuelan Equine - diagnosis; Encephalomyelitis, Venezuelan Equine - virology; experimental infection; Fibroblasts - pathology; Fibroblasts - virology; Humans; laboratory animals; Macaca mulatta; Mice; pcr; Rats; Reagent Kits, Diagnostic - standards; Reverse Transcriptase Polymerase Chain Reaction - methods; reverse transcription; rna; RNA, Viral - genetics; RNA, Viral - isolation & purification; Sindbis Virus - genetics; Sindbis Virus - isolation & purification; vee virus; West Nile Fever - diagnosis; West Nile Fever - virology; West Nile virus - genetics; West Nile virus - isolation & purification
• ISSN: 0372-9311; 2686-7613

Detection-and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR). VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses were studied. Cell culture of chicken fibroblasts, outbred mice and rats, Javanese macaques were used in the experiments. Biological activity determination of the running culture of causative agents used in the experiments was carried out by negative colony method in monolayer cell culture under agar coating. and using intra-cerebral infection of mice. Reagent kits developed in the 48th Central Research Institute and Institute of Analytical Instrument Engineering were used during execution of experiments of VEE virus RNA detection by RT-PCR and rRT-PCR. VEE virus was detected in biological samples by various methods. Data from RT-PCR and rRT-PCR are in accordance with the results of virus detection in samples using sensitive animals. Use of molecular-diagnostics methods for detection in biological samples of a causative agent of a dangerous infectious disease is important for procuring biological safety of Russian Federation.