Silencing and nuclear repositioning of the lambda5 gene locus at the pre-B cell stage requires Aiolos and OBF-1

The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a blo...

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Published inPloS one Vol. 3; no. 10; p. e3568
Main Authors Karnowski, Alexander, Cao, Chun, Matthias, Gabriele, Carotta, Sebastian, Corcoran, Lynn M, Martensson, Inga-Lill, Skok, Jane A, Matthias, Patrick
Format Journal Article
LanguageEnglish
Published United States Public Library of Science (PLoS) 2008
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Abstract The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a block is uncovered at the transition between pre-B and immature B cells, indicating that these proteins exert a critical function in developing B lymphocytes. In mice deficient for Aiolos and OBF-1, the numbers of immature B cells are reduced, small pre-BII cells are increased and a significant impairment in immunoglobulin light chain DNA rearrangement is observed. We identified genes whose expression is deregulated in the pre-B cell compartment of these mice. In particular, we found that components of the pre-BCR, such as the surrogate light chain genes lambda5 and VpreB, fail to be efficiently silenced in double-mutant mice. Strikingly, developmentally regulated nuclear repositioning of the lambda5 gene is impaired in pre-B cells lacking OBF-1 and Aiolos. These studies uncover a novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function.
AbstractList The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a block is uncovered at the transition between pre-B and immature B cells, indicating that these proteins exert a critical function in developing B lymphocytes. In mice deficient for Aiolos and OBF-1, the numbers of immature B cells are reduced, small pre-BII cells are increased and a significant impairment in immunoglobulin light chain DNA rearrangement is observed. We identified genes whose expression is deregulated in the pre-B cell compartment of these mice. In particular, we found that components of the pre-BCR, such as the surrogate light chain genes lambda5 and VpreB, fail to be efficiently silenced in double-mutant mice. Strikingly, developmentally regulated nuclear repositioning of the lambda5 gene is impaired in pre-B cells lacking OBF-1 and Aiolos. These studies uncover a novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function.
Author Carotta, Sebastian
Cao, Chun
Karnowski, Alexander
Martensson, Inga-Lill
Matthias, Patrick
Corcoran, Lynn M
Skok, Jane A
Matthias, Gabriele
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SubjectTerms Animals
Bone Marrow Cells - metabolism
Bone Marrow Cells - physiology
Cell Differentiation - genetics
Cell Differentiation - immunology
Cell Nucleus - genetics
Cells, Cultured
Gene Expression Profiling
Gene Expression Regulation - immunology
Gene Order - physiology
Gene Rearrangement, B-Lymphocyte, Light Chain
Gene Silencing - immunology
Immunoglobulin lambda-Chains - genetics
Immunoglobulin lambda-Chains - metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Oligonucleotide Array Sequence Analysis
Precursor Cells, B-Lymphoid - metabolism
Precursor Cells, B-Lymphoid - physiology
Trans-Activators - genetics
Trans-Activators - physiology
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Title Silencing and nuclear repositioning of the lambda5 gene locus at the pre-B cell stage requires Aiolos and OBF-1
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