Genotyping Klebsiella pneumoniae isolated from hepatic abscesses in three patients from Bogota, Colombia
Pyogenic liver abscess caused by Klebsiella pneumoniae represents an ever increasing entity which has mainly been described as occurring in Asia, even though, on a smaller scale, cases are being more frequently described from the USA and Europe, 13% overall mortality being reached worldwide. Affecte...
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Published in | Revista ciencias de la salud Vol. 11; no. 1; pp. 135 - 144 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Portuguese Spanish |
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Editorial Universidad del Rosario
01.04.2013
Universidad Colegio Mayor Nuestra Señora del Rosario |
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Abstract | Pyogenic liver abscess caused by Klebsiella pneumoniae represents an ever increasing entity which has mainly been described as occurring in Asia, even though, on a smaller scale, cases are being more frequently described from the USA and Europe, 13% overall mortality being reached worldwide. Affected patients are severely sick, suffering from fever, sweating, having increased acute phase reactants and risk factors such as Diabetes Mellitus, alcoholism and the inherent characteristics of the bacteria causing the disease. Objective: in this work we used a Multilocus Sequencing Typing (MLST), a nucleotide sequence-based method in order to characterize the genetic relationships among bacterial isolates. Materials and methods: the report is focused on three cases involving patients suffering from pyogenic liver abscess caused by Klebsiella pneumoniae in two hospitals in Bogota, Colombia, where phenotyping and hypermucoviscosity studies were carried out, as well as the genotyping of cultured Klebsiella isolates. Results: it was found that the isolated microorganism in cases I and II corresponded to the same K. pneumoniae strain, having 100% sequence identity for the 5 genes being studied while the strain in Case III was genotypically different. Conclusion: it is important to carry out multidisciplinary studies allowing all pyogenic liver abscess cases reported in Colombia to be complied to ascertain the frequency of microorganisms causing this pathology in our country, as well as a genotyping study of different K. pneumoniae strains to compare them and confirm clonal and pathogenicity relationships through housekeeping gene analysis. |
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AbstractList | Pyogenic liver abscess caused by Klebsiella pneumoniae represents an ever increasing entity which has mainly been described as occurring in Asia, even though, on a smaller scale, cases are being more frequently described from the USA and Europe, 13% overall mortality being reached worldwide. Affected patients are severely sick, suffering from fever, sweating, having increased acute phase reactants and risk factors such as Diabetes Mellitus, alcoholism and the inherent characteristics of the bacteria causing the disease. Objective: in this work we used a Multilocus Sequencing Typing (MLST), a nucleotide sequence-based method in order to characterize the genetic relationships among bacterial isolates. Materials and methods: the report is focused on three cases involving patients suffering from pyogenic liver abscess caused by Klebsiella pneumoniae in two hospitals in Bogota, Colombia, where phenotyping and hypermucoviscosity studies were carried out, as well as the genotyping of cultured Klebsiella isolates. Reults: it was found that the isolated microorganism in cases I and II corresponded to the same K. pneumoniae strain, having 100% sequence identity for the 5 genes being studied while the strain in Case III was genotypically different. Conclusion: it is important to carry out multidisciplinary studies allowing all pyogenic liver abscess cases reported in Colombia to be complied to ascertain the frequency of microorganisms causing this pathology in our country, as well as a genotyping study of different K. pneumoniae strains to compare them and confirm clonal and pathogenicity relationships through housekeeping gene analysis. Pyogenic liver abscess caused by Klebsiella pneumoniae represents an ever increasing entity which has mainly been described as occurring in Asia, even though, on a smaller scale, cases are being more frequently described from the USA and Europe, 13% overall mortality being reached worldwide. Affected patients are severely sick, suffering from fever, sweating, having increased acute phase reactants and risk factors such as Diabetes Mellitus, alcoholism and the inherent characteristics of the bacteria causing the disease. Objective: in this work we used a Multilocus Sequencing Typing (MLST), a nucleotide sequence-based method in order to characterize the genetic relationships among bacterial isolates. Materials and methods: the report is focused on three cases involving patients suffering from pyogenic liver abscess caused by Klebsiella pneumoniae in two hospitals in Bogota, Colombia, where phenotyping and hypermucoviscosity studies were carried out, as well as the genotyping of cultured Klebsiella isolates. Results: it was found that the isolated microorganism in cases I and II corresponded to the same K. pneumoniae strain, having 100% sequence identity for the 5 genes being studied while the strain in Case III was genotypically different. Conclusion: it is important to carry out multidisciplinary studies allowing all pyogenic liver abscess cases reported in Colombia to be complied to ascertain the frequency of microorganisms causing this pathology in our country, as well as a genotyping study of different K. pneumoniae strains to compare them and confirm clonal and pathogenicity relationships through housekeeping gene analysis. |
Author | Gómez, Arley Amado, Danys Ríos, Dora I. Tobón, Luisa F. Pardo, Juan M. |
AuthorAffiliation | Universidad del Rosario |
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Author_xml | – sequence: 1 givenname: Dora I. surname: Ríos fullname: Ríos, Dora I. organization: Universidad del Rosario – sequence: 2 givenname: Luisa F. surname: Tobón fullname: Tobón, Luisa F. organization: Universidad del Rosario – sequence: 3 givenname: Danys surname: Amado fullname: Amado, Danys organization: Universidad del Rosario – sequence: 4 givenname: Juan M. surname: Pardo fullname: Pardo, Juan M. organization: Universidad del Rosario – sequence: 5 givenname: Arley surname: Gómez fullname: Gómez, Arley organization: Universidad del Rosario |
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DocumentTitleAlternate | Genotipificação de Klebsiella pneumoniae isolada de abscessos hepáticos de pacientes de Bogotá, Colômbia |
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Keywords | abscesso hepático K1 capsular serotype hypermucoviscosity sorotipo capsular K1 hipermucoviscosidad piógeno absceso hepático tipificação por sequências de multilocus piogênico multi-locus sequence typing serotipo capsular K1 tipificación por secuencias de multilocus liver abscess hiper-mucoviscosidad pyogenic Klebsiella pneumoniae |
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References_xml | – volume: 4 start-page: 107 issue: 2 year: 2013 end-page: 18 article-title: Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: A new and dangerous breed publication-title: Virulence contributor: fullname: Shon, AS; Bajwa, RP; Russo, TA – volume: 62 start-page: 1 issue: 1 year: 2008 end-page: 6 article-title: Comparison of prevalence of virulence factors for Klebsiella pneumoniae liver abscesses between isolates with capsular K1/K2 and non-K1/ K2 serotypes publication-title: Diagn Microbiol Infect Dis contributor: fullname: Yu, W-L; Ko, W-C; Cheng, K-C; Lee, C-C; Lai, C-C; Chuang, Y-C – volume: 52 start-page: 79 issue: 2 year: 2005 end-page: 84 article-title: Risk factors for spontaneous rupture of liver abscess caused by Klebsiella pneumoniae publication-title: Diagn Microbiol Infect Dis contributor: fullname: Lee, C-H; Leu, H-S; Wu, T-S; Su, L-H; Liu, J-W – volume: 136 start-page: 119 issue: 7-8 year: 2006 end-page: 26 article-title: Risk factors for developing metastatic infection from pyogenic liver abscesses publication-title: Swiss Med Wkly contributor: fullname: Chen, S-C; Lee, Y-T; Lai, K-C; Cheng, K-S; Jeng, L-B; Wu, W-Y – volume: 8 start-page: 160 issue: 2 year: 2002 end-page: 6 article-title: Community-acquired Klebsiella pneumoniae bacteremia: Global differences in clinical patterns publication-title: Emerg Infect Dis contributor: fullname: Ko, W-C; Paterson, DL; Sagnimeni, AJ; Hansen, DS; Von Gottberg, A; Mohapatra, S – volume: 14 start-page: 1592 issue: 10 year: 2008 end-page: 600 article-title: Pyogenic liver abscess as endemic disease, Taiwan publication-title: Emerg Infect Dis contributor: fullname: Tsai F-CHuang, YT; Chang, LY; Wang, JT – volume: 11 start-page: 589 issue: 4 year: 1998 end-page: 603 article-title: Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors publication-title: Clin Microbiol Rev contributor: fullname: Podschun, R; Ullmann, U – volume: 43 start-page: 991 issue: 2 year: 2005 end-page: 2 article-title: Liver abscess caused by mag A+ Klebsiella pneumoniae in North America publication-title: J Clin Microbiol contributor: fullname: Fang, FC; Sandler, N; Libby, SJ – volume: 12 start-page: 881 issue: 11 year: 2012 end-page: 7 article-title: Klebsiella pneumoniae liver abscess: a new invasive syndrome publication-title: Lancet Infect Dis contributor: fullname: Siu, LK; Yeh, KM; Lin, JC; Fung, CP; Chang, FY – volume: 26 start-page: 528 issue: 4 year: 2006 end-page: 37 article-title: Genes involucrados en la biogénesis de fimbrias afectan la formación de biopelículas por parte de Klebsiella pneumoniae publication-title: Biomedica contributor: fullname: Suescún, AV; Cubillos, JR; Zambrano, MM – volume: 45 start-page: 466 issue: 2 year: 2007 end-page: 71 article-title: Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in Singapore and Taiwan publication-title: J Clin Microbiol contributor: fullname: Yeh, K-M; Kurup, A; Siu, LK; Koh, YL; Fung, C-P; Lin, J-C – volume: 30 start-page: 385 issue: 5 year: 2007 end-page: 9 article-title: The changing face of Klebsiella pneumoniae infections in the community publication-title: Int J Antimicrob Agents contributor: fullname: Keynan, Y; Rubinstein, E – volume: 2 start-page: 442 issue: 6 year: 2007 end-page: 4 article-title: Klebsiella pneumoniae endophthalmitis with associated hepatic abscess publication-title: J Hosp Med contributor: fullname: Connell, NT; Thomas, IA; Sabharwal, AD; Gelbard, MA – volume: 41 start-page: 559 issue: 2 year: 2013 end-page: 63 article-title: Survey of Klebsiella pneumoniae bacteraemia in two South Australian hospitals and detection of hypermucoviscous phenotype and mag A/rmp A genotypes in K. pneumoniae isolates publication-title: Infection contributor: fullname: Chang, L; Bastian, I; Warner, M – volume: 42 start-page: 1359 issue: 10 year: 2006 end-page: 61 article-title: Hypermucoviscosity: an extremely sticky phenotype of Klebsiella pneumoniae associated with emerging destructive tissue abscess syndrome publication-title: Clin Infect Dis contributor: fullname: Kawai, T. – volume: 100 start-page: 322 issue: 2 year: 2005 end-page: 31 article-title: Pyogenic liver abscess with a focus on Klebsiella pneumoniae as a primary pathogen: an emerging disease with unique clinical characteristics publication-title: Am J Gastroenterol contributor: fullname: Lederman, ER; Crum, NF – volume: 45 start-page: e25 issue: 3 year: 2007 end-page: 8 article-title: Invasive Klebsiella pneumoniae syndrome in North America publication-title: Clin Infect Dis contributor: fullname: Nadasy, KA; Domiati-Saad, R; Tribble, MA |
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SubjectTerms | hypermucoviscosity K1 capsular serotype Klebsiella pneumoniae liver abscess multi-locus sequence typing PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH pyogenic |
Title | Genotyping Klebsiella pneumoniae isolated from hepatic abscesses in three patients from Bogota, Colombia |
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