幽门螺杆菌及其vacA 基因亚型和cagA 基因与胃上皮HLA-DR 抗原表达的关系

摘　要:【目的】探讨幽门螺杆菌及其空泡毒素基因(vacA)亚型、细胞毒素相关基因(cagA)与胃上皮HLA-DR 抗原表达 间的关系。【方法】①自39 例幽门螺杆菌阳性患者中分离培养出39 株幽门螺杆菌菌株, 采用PCR 方法鉴定39 株菌株的cagA 及vacA 亚型;②采用HLA-DR 小鼠抗人单克隆抗体, 对上述已行cagA 及vacA 亚型鉴定的幽门螺杆菌阳性患者及22 例阴 性患者的胃窦活检标本行免疫组化染色。【结果】①幽门螺杆菌阳性患者胃上皮HLA-DR 表达较阴性患者更显著;②幽门螺 杆菌定植密度与胃上皮HLA-DR 抗原表达程度间存在正相关;③感染cagA+菌株患者较感染cag...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 23
Main Authors 何　瑶, 胡品津, 何兴祥, 曾志荣, 陈　为, 彭晓忠
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2002
Subjects
cagA /HLA-DR
螺杆菌/ 幽门/vacA
Online AccessGet full text
ISSN1672-3554
DOI10.3321/j.issn:1672-3554.2002.01.016

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Abstract 摘　要:【目的】探讨幽门螺杆菌及其空泡毒素基因(vacA)亚型、细胞毒素相关基因(cagA)与胃上皮HLA-DR 抗原表达 间的关系。【方法】①自39 例幽门螺杆菌阳性患者中分离培养出39 株幽门螺杆菌菌株, 采用PCR 方法鉴定39 株菌株的cagA 及vacA 亚型;②采用HLA-DR 小鼠抗人单克隆抗体, 对上述已行cagA 及vacA 亚型鉴定的幽门螺杆菌阳性患者及22 例阴 性患者的胃窦活检标本行免疫组化染色。【结果】①幽门螺杆菌阳性患者胃上皮HLA-DR 表达较阴性患者更显著;②幽门螺 杆菌定植密度与胃上皮HLA-DR 抗原表达程度间存在正相关;③感染cagA+菌株患者较感染cagA -株者胃上皮HLA-DR 抗 原表达更明显;④本研究中v acA s1a/ m2 亚型占90 %, 故未对不同vacA 亚型与胃上皮HLA-DR 表达间的关系进行统计分析。 【结论】①幽门螺杆菌感染可诱导胃上皮HLA-DR 抗原异常表达;②cagA+菌株可能通过胃上皮HLA-Ⅱ 类分子介导而与宿主 发生更为密切的相互作用, 从而较cagA-菌株引起更为显著的胃上皮损伤。
AbstractList 摘　要:【目的】探讨幽门螺杆菌及其空泡毒素基因(vacA)亚型、细胞毒素相关基因(cagA)与胃上皮HLA-DR 抗原表达 间的关系。【方法】①自39 例幽门螺杆菌阳性患者中分离培养出39 株幽门螺杆菌菌株, 采用PCR 方法鉴定39 株菌株的cagA 及vacA 亚型;②采用HLA-DR 小鼠抗人单克隆抗体, 对上述已行cagA 及vacA 亚型鉴定的幽门螺杆菌阳性患者及22 例阴 性患者的胃窦活检标本行免疫组化染色。【结果】①幽门螺杆菌阳性患者胃上皮HLA-DR 表达较阴性患者更显著;②幽门螺 杆菌定植密度与胃上皮HLA-DR 抗原表达程度间存在正相关;③感染cagA+菌株患者较感染cagA -株者胃上皮HLA-DR 抗 原表达更明显;④本研究中v acA s1a/ m2 亚型占90 %, 故未对不同vacA 亚型与胃上皮HLA-DR 表达间的关系进行统计分析。 【结论】①幽门螺杆菌感染可诱导胃上皮HLA-DR 抗原异常表达;②cagA+菌株可能通过胃上皮HLA-Ⅱ 类分子介导而与宿主 发生更为密切的相互作用, 从而较cagA-菌株引起更为显著的胃上皮损伤。
Author 陈　为
彭晓忠
何兴祥
何　瑶
胡品津
曾志荣
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螺杆菌/ 幽门/vacA
Title 幽门螺杆菌及其vacA 基因亚型和cagA 基因与胃上皮HLA-DR 抗原表达的关系
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