逆转录病毒介导的vIL-10 表达对小鼠MLC和心脏移植存活期的影响

【目的】探讨逆转录病毒介导的病毒白介素10(vIL-10)基因表达对小鼠混合淋巴细胞培养(MLC)反应和心脏移植 存活期的影响。【方法】将vIL-10 cDNA 克隆到逆转录病毒载体pLXSN, 取vIL-10 重组逆转录病毒上清加入BABL/ c 和CBA/ J 混合淋巴细胞中培养, 液闪测量。在新生CBA/ J 小鼠心脏移植时, 将vIL-10 上清10 μL(1 000 pfu)注入移植心脏内, 观察其对心 脏移植存活期的影响。【结果】vIL-10 抑制小鼠混合淋巴细胞反应(MLR), 其抑制效应随vIL-10 病毒上清的增加而增加, 逆转 导病毒介导的vIL-10 表达使小鼠心脏移植存...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 21
Main Authors 钟建辉, 杨彦强, 陈永雄, 黄凌虹, 余学清
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2000
Online AccessGet full text
ISSN1672-3554
DOI10.3321/j.issn:1672-3554.2000.03.015

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Abstract 【目的】探讨逆转录病毒介导的病毒白介素10(vIL-10)基因表达对小鼠混合淋巴细胞培养(MLC)反应和心脏移植 存活期的影响。【方法】将vIL-10 cDNA 克隆到逆转录病毒载体pLXSN, 取vIL-10 重组逆转录病毒上清加入BABL/ c 和CBA/ J 混合淋巴细胞中培养, 液闪测量。在新生CBA/ J 小鼠心脏移植时, 将vIL-10 上清10 μL(1 000 pfu)注入移植心脏内, 观察其对心 脏移植存活期的影响。【结果】vIL-10 抑制小鼠混合淋巴细胞反应(MLR), 其抑制效应随vIL-10 病毒上清的增加而增加, 逆转 导病毒介导的vIL-10 表达使小鼠心脏移植存活期从(11±1)d 延长至(28±3)d (P <0.01)。【结论】逆转录病毒介导的vLL -10 表达抑制小鼠MLR, 使小鼠心脏移植存活期明显延长。
AbstractList 【目的】探讨逆转录病毒介导的病毒白介素10(vIL-10)基因表达对小鼠混合淋巴细胞培养(MLC)反应和心脏移植 存活期的影响。【方法】将vIL-10 cDNA 克隆到逆转录病毒载体pLXSN, 取vIL-10 重组逆转录病毒上清加入BABL/ c 和CBA/ J 混合淋巴细胞中培养, 液闪测量。在新生CBA/ J 小鼠心脏移植时, 将vIL-10 上清10 μL(1 000 pfu)注入移植心脏内, 观察其对心 脏移植存活期的影响。【结果】vIL-10 抑制小鼠混合淋巴细胞反应(MLR), 其抑制效应随vIL-10 病毒上清的增加而增加, 逆转 导病毒介导的vIL-10 表达使小鼠心脏移植存活期从(11±1)d 延长至(28±3)d (P <0.01)。【结论】逆转录病毒介导的vLL -10 表达抑制小鼠MLR, 使小鼠心脏移植存活期明显延长。
Author 杨彦强
陈永雄
余学清
钟建辉
黄凌虹
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Snippet 【目的】探讨逆转录病毒介导的病毒白介素10(vIL-10)基因表达对小鼠混合淋巴细胞培养(MLC)反应和心脏移植 存活期的影响。【方法】将vIL-10 cDNA 克隆到逆转录病毒载体pLXSN, 取vIL-10 重组逆转录病毒上清加入BABL/ c 和CBA/ J 混合淋巴细胞中培养, 液闪测量。在新生CBA/ J...
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Title 逆转录病毒介导的vIL-10 表达对小鼠MLC和心脏移植存活期的影响
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