Identification of factors required for m 6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI

• Published in: The New phytologist Vol. 215; no. 1; pp. 157 - 172
• Main Authors: Růžička, Kamil, Zhang, Mi, Campilho, Ana, Bodi, Zsuzsanna, Kashif, Muhammad, Saleh, Mária, Eeckhout, Dominique, El-Showk, Sedeer, Li, Hongying, Zhong, Silin, De Jaeger, Geert, Mongan, Nigel P, Hejátko, Jan, Helariutta, Ykä, Fray, Rupert G
• Format: Journal Article
• Language: English
• Published: England 01.07.2017
• Subjects: Adenosine - metabolism; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis Proteins - physiology; Conserved Sequence; Methylation; Methyltransferases - genetics; Methyltransferases - metabolism; Methyltransferases - physiology; Plants, Genetically Modified - metabolism; RNA, Messenger - metabolism; Sequence Alignment; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitin-Protein Ligases - physiology; N6-adenosine methylation (m6A); Arabidopsis; VIRILIZER; protoxylem; HAKAI; mRNA methylation

N6-adenosine methylation (m A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m A writer prote...