Identification of factors required for m 6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI

N6-adenosine methylation (m A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m A writer prote...

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Published in	The New phytologist Vol. 215; no. 1; pp. 157 - 172
Main Authors	Růžička, Kamil, Zhang, Mi, Campilho, Ana, Bodi, Zsuzsanna, Kashif, Muhammad, Saleh, Mária, Eeckhout, Dominique, El-Showk, Sedeer, Li, Hongying, Zhong, Silin, De Jaeger, Geert, Mongan, Nigel P, Hejátko, Jan, Helariutta, Ykä, Fray, Rupert G
Format	Journal Article
Language	English
Published	England 01.07.2017
Subjects	Adenosine - metabolism Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Conserved Sequence Methylation Methyltransferases - genetics Methyltransferases - metabolism Methyltransferases - physiology Plants, Genetically Modified - metabolism RNA, Messenger - metabolism Sequence Alignment Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism Ubiquitin-Protein Ligases - physiology N6-adenosine methylation (m6A) Arabidopsis VIRILIZER protoxylem HAKAI mRNA methylation
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Abstract	N6-adenosine methylation (m A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m A writer proteins in Arabidopsis thaliana. The components required for m A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.
AbstractList	Summary N 6‐adenosine methylation (m 6 A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m 6 A writer proteins in Arabidopsis thaliana . The components required for m 6 A in Arabidopsis included MTA , MTB , FIP 37, VIRILIZER and the E3 ubiquitin ligase HAKAI . Downregulation of these proteins led to reduced relative m 6 A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m 6 A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m 6 A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences. N6-adenosine methylation (m A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m A writer proteins in Arabidopsis thaliana. The components required for m A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.
Author	Li, Hongying Zhang, Mi Zhong, Silin Saleh, Mária Fray, Rupert G Bodi, Zsuzsanna Helariutta, Ykä Campilho, Ana Eeckhout, Dominique Hejátko, Jan Kashif, Muhammad De Jaeger, Geert El-Showk, Sedeer Mongan, Nigel P Růžička, Kamil
Author_xml	– sequence: 1 givenname: Kamil surname: Růžička fullname: Růžička, Kamil organization: Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland – sequence: 2 givenname: Mi surname: Zhang fullname: Zhang, Mi organization: Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK – sequence: 3 givenname: Ana surname: Campilho fullname: Campilho, Ana organization: Research Center in Biodiversity and Genetic Resources, University of Porto, 4485-661 Vairão, Portugal – sequence: 4 givenname: Zsuzsanna surname: Bodi fullname: Bodi, Zsuzsanna organization: Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK – sequence: 5 givenname: Muhammad surname: Kashif fullname: Kashif, Muhammad organization: Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland – sequence: 6 givenname: Mária surname: Saleh fullname: Saleh, Mária organization: Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 62500, Brno, Czech Republic – sequence: 7 givenname: Dominique surname: Eeckhout fullname: Eeckhout, Dominique organization: Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium – sequence: 8 givenname: Sedeer surname: El-Showk fullname: El-Showk, Sedeer organization: Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland – sequence: 9 givenname: Hongying surname: Li fullname: Li, Hongying organization: Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, Shanxi, 030031, China – sequence: 10 givenname: Silin surname: Zhong fullname: Zhong, Silin organization: The State Key Laboratory of Agrobiotechnology, The School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China – sequence: 11 givenname: Geert surname: De Jaeger fullname: De Jaeger, Geert organization: Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium – sequence: 12 givenname: Nigel P surname: Mongan fullname: Mongan, Nigel P organization: School of Veterinary Medicine and Sciences, University of Nottingham, Sutton Bonington, Loughborough, LE12 5RD, UK – sequence: 13 givenname: Jan surname: Hejátko fullname: Hejátko, Jan organization: Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 62500, Brno, Czech Republic – sequence: 14 givenname: Ykä surname: Helariutta fullname: Helariutta, Ykä organization: Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR, UK – sequence: 15 givenname: Rupert G surname: Fray fullname: Fray, Rupert G organization: Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/28503769$$D View this record in MEDLINE/PubMed
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Keywords	N6-adenosine methylation (m6A) Arabidopsis VIRILIZER protoxylem HAKAI mRNA methylation
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Snippet	N6-adenosine methylation (m A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are... Summary N 6‐adenosine methylation (m 6 A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this...
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SubjectTerms	Adenosine - metabolism Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Conserved Sequence Methylation Methyltransferases - genetics Methyltransferases - metabolism Methyltransferases - physiology Plants, Genetically Modified - metabolism RNA, Messenger - metabolism Sequence Alignment Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism Ubiquitin-Protein Ligases - physiology
Title	Identification of factors required for m 6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
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