Streptomyces castrisilvae sp. nov. and Streptomyces glycanivorans sp. nov., novel soil streptomycetes metabolizing mutan and alternan
Six bacterial strains, Mut1 , Mut2, Alt1, Alt2, Alt3 , and Alt4, were isolated from soil samples collected in parks in Gothenburg, Sweden, based on their ability to utilize the insoluble polysaccharides α-1,3-glucan (mutan; Mut strains) or the mixed-linkage α-1,3/α-1,6-glucan (alternan; Alt strains)...
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Published in | International journal of systematic and evolutionary microbiology Vol. 74; no. 9 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.09.2024
|
Subjects | |
Online Access | Get more information |
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Summary: | Six bacterial strains, Mut1
, Mut2, Alt1, Alt2, Alt3
, and Alt4, were isolated from soil samples collected in parks in Gothenburg, Sweden, based on their ability to utilize the insoluble polysaccharides α-1,3-glucan (mutan; Mut strains) or the mixed-linkage α-1,3/α-1,6-glucan (alternan; Alt strains). Analysis of 16S rRNA gene sequences identified all strains as members of the genus
. The genomes of the strains were sequenced and subsequent phylogenetic analyses identified Mut2 as a strain of
and Alt1, Alt2 and Alt4 as strains of
while Mut1
and Alt3
were most closely related to the type strains
NBRC 101007
and
NRRL ISP-5137
, respectively. Comprehensive genomic and biochemical characterizations were conducted, highlighting typical features of
, such as large genomes (8.0-9.6 Mb) with high G+C content (70.5-72.0%). All six strains also encode a wide repertoire of putative carbohydrate-active enzymes, indicating a capability to utilize various complex polysaccharides as carbon sources such as starch, mutan, and cellulose, which was confirmed experimentally. Based on phylogenetic and phenotypic characterization, our study suggests that strains Mut1
and Alt3
represent novel species in the genus
for which the names
sp. nov. and
sp. nov. are proposed, with strains Mut1
(=DSM 117248
=CCUG 77596
) and Alt3
(=DSM 117252
=CCUG 77600
) representing the respective type strains. |
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ISSN: | 1466-5034 |
DOI: | 10.1099/ijsem.0.006514 |