Analysis of Genetic Relationship among Boehmeria Varieties Using cpSSR Markers

By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism r...

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Published inAgricultural biotechnology (Pawtucket, R.I.) Vol. 4; no. 3; pp. 24 - 26
Main Authors Chen, Ping, Yu, Chunming, Wang, Yanzhou, Chen, Jikang, Lu, Lingxiao, Xiong, Heping
Format Journal Article
LanguageEnglish
Published Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.06.2015
Subjects
Boehmeria
Chloroplasts
Cluster analysis
Evolution & development
Genomes
Morphology
Phylogenetics
Polymorphism
SSR引物
SSR标记
Tobacco
品种间
多态性位点
实验材料
苎麻
遗传关系
China
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Abstract By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.
AbstractList By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.
By using cpSSR (chloroplast microsatellites) markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of cluster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.
Author Ping CHEN Chunming YU Yanzhou WANG Jikang CHEN Lingxiao LU Heping XIONG
AuthorAffiliation Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
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Notes Boehmeria nivea （L.） Gaudich. ; Genetic relationship ; cpSSR
By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.
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Snippet By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with...
By using cpSSR (chloroplast microsatellites) markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with...
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SubjectTerms Boehmeria
Chloroplasts
Cluster analysis
Evolution & development
Genomes
Morphology
Phylogenetics
Polymorphism
SSR引物
SSR标记
Tobacco
品种间
多态性位点
实验材料
苎麻
遗传关系
Title Analysis of Genetic Relationship among Boehmeria Varieties Using cpSSR Markers
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