TMOD-23. MODELING PEDIATRIC BRAIN CANCER WITH HUMAN ORGANOIDS
Abstract Among children/infant brain tumors, Medulloblastoma (MB) is the most common and stands as a cause for a high percentage of morbidity and mortality among patients During the past few years, studies on human MB have uncovered the existence of four major MB groups: WNT, SHH, Group3 and Group4....
Saved in:
| Published in | Neuro-oncology (Charlottesville, Va.) Vol. 23; no. Supplement_6; p. vi220 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
12.11.2021
|
| Online Access | Get full text |
Cover
Loading…
| Summary: | Abstract
Among children/infant brain tumors, Medulloblastoma (MB) is the most common and stands as a cause for a high percentage of morbidity and mortality among patients During the past few years, studies on human MB have uncovered the existence of four major MB groups: WNT, SHH, Group3 and Group4. Patients with Group3 MB currently have the worst outcome among the four groups, and nearly 50% are metastatic at the time of diagnosis. In the last 3 years in our laboratory, we have developed a novel pediatric Medulloblastoma organoid model. We generated human iPSC-derived cancer organoids upon c-MYC/OTX2 and C-MYC/Gfi1 overexpression, mimicking human MB genetic alterations. Furthermore, the use of DNA methylation signature in combination with MB-specific markers analysis indicates that our organoid-based cancer model recapitulates several features of human MB. Now, we are taking advantage of this technology to produce novel brain cancer organoids that we are using to address cancer biology questions. |
|---|---|
| ISSN: | 1522-8517 1523-5866 |
| DOI: | 10.1093/neuonc/noab196.884 |