Establishment of Multiplex RT-PCR Detection System for Three Viruses in Freesia

The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in fre...

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Published inPlant Diseases and Pests(植物病虫害研究：英文版) Vol. 4; no. 1; pp. 33 - 35
Main Authors Ronghui, Fan, Minling, Huang, Jianshe, Wu, Yuanhua, Luo
Format Journal Article
LanguageEnglish
Published Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.02.2013
Subjects
Bean yellow mosaic virus
Cucumber mosaic virus
Cytomegalovirus
Freesia
Freesia mosaic virus
GenBank
多重PCR
多重RT-PCR
小苍兰
检测系统
病毒外壳蛋白
黄瓜花叶病毒
黄色花叶病毒
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Summary:The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in freesia was developed. Three specific fragments were simultaneously amplified in a single PCR reaction. Their lengths were determined to be 340,628 and 212 bp, respec-tively. The sequence analysis indicated that three viruses shared at least 97% of homology with reference sequence. Sensitivity test showed that these three viruses could be detected out in the infected plant tissue greater than 10^-2 mg.
Bibliography:The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in freesia was developed. Three specific fragments were simultaneously amplified in a single PCR reaction. Their lengths were determined to be 340,628 and 212 bp, respec-tively. The sequence analysis indicated that three viruses shared at least 97% of homology with reference sequence. Sensitivity test showed that these three viruses could be detected out in the infected plant tissue greater than 10^-2 mg.
Fan Ronghui, Huang Minling , Wu Jianshe, Luo Yuanhua（1. Institute of Crop Science, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2. Flowers Research Center, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 3. Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou 350013, China）
Freesia; Virus; Freesia mosaic virus ; Cucumber mosaic virus ; Bean yellow mosaic virus; Multiplex PCR
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ISSN:2152-3932