Reconstitution of a microtubule plus-end tracking system in vitro

• Published in: Nature Vol. 450; no. 7172; pp. 1100 - 1105
• Main Authors: Bieling, Peter, Brunner, Damian, Munteanu, E. Laura, Surrey, Thomas, Sandblad, Linda, Dogterom, Marileen, Schek, Henry, Laan, Liedewij
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 13.12.2007; Nature Publishing Group
• Subjects: Biological and medical sciences; Cell structures and functions; Cell-Free System; Cellular biology; Cytoskeleton, cytoplasm. Intracellular movements; Fluorescence; Fluorescence microscopy; Fundamental and applied biological sciences. Psychology; Heat-Shock Proteins - metabolism; Intermediate Filament Proteins - metabolism; Microscopy; Microscopy, Fluorescence; Microtubule-Associated Proteins - metabolism; Microtubules - chemistry; Microtubules - metabolism; Molecular and cellular biology; Molecular biology; Proteins; Schizosaccharomyces - chemistry; Schizosaccharomyces - cytology; Schizosaccharomyces pombe; Schizosaccharomyces pombe Proteins - metabolism; Yeast; Yeasts; Ascomycota; Fungi; Schizosaccharomyces pombe; Cytoskeleton; Molecular interaction; End; Reconstitution; Microtubule; In vitro

The microtubule cytoskeleton is essential to cell morphogenesis. Growing microtubule plus ends have emerged as dynamic regulatory sites in which specialized proteins, called plus-end-binding proteins (+TIPs), bind and regulate the proper functioning of microtubules. However, the molecular mechanism...