Cryobiological Characteristics of L-proline in Mammalian Oocyte Cryopreservation

• Published in: Chinese medical journal Vol. 129; no. 16; pp. 1963 - 1968
• Main Authors: Zhang, Lu, Xue, Xu, Yan, Jie, Yan, Li-Ying, Jin, Xiao-Hu, Zhu, Xiao-Hui, He, Zhi-Zhu, Liu, Jing, Li, Rong, Qiao, Jie
• Format: Journal Article
• Language: English
• Published: China Wolters Kluwer - Medknow Publications 20.08.2016; Medknow Publications and Media Pvt. Ltd; Lippincott Williams & Wilkins Ovid Technologies; Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China%Beijing Key Laboratory of Cryo-Biomedical Engineering and Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China%Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer
• Subjects: Analysis; Animals; Biotechnology industry; Conflicts of interest; Cryopreservation; Cryopreservation - methods; Cryoprotective Agentant; Mouse Oocyte; Vitrification; Cryoprotective Agents - pharmacology; DNA methylation; Efficiency; Female; Fertilization in Vitro; Gene expression; Hydrogen-Ion Concentration; Laboratory animals; Male; Mice; Oocytes; Oocytes - drug effects; Original; Osmotic Pressure; Proline; Proline - pharmacology; Raman spectroscopy; Spectrum analysis; Spectrum Analysis, Raman; Sucrose; Vitrification; China; Mouse Oocyte; Cryoprotective Agentant; Vitrification

Background： L-proline is a natural, nontoxic cryoprotectant that helps cells and tissues to tolerate freezing in a variety of plants and animals. The use of L-proline in mammalian oocyte cryopreservation is rare. In this study, we explored the cryobiological characteristics of L-proline and evaluate...