Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation

• Published in: PLoS medicine Vol. 16; no. 4; p. e1002794
• Main Authors: Colman, Rebecca E., Mace, Aurélien, Seifert, Marva, Hetzel, Jonathan, Mshaiel, Haifa, Suresh, Anita, Lemmer, Darrin, Engelthaler, David M., Catanzaro, Donald G., Young, Amanda G., Denkinger, Claudia M., Rodwell, Timothy C.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.04.2019; Public Library of Science (PLoS)
• Subjects: Antitubercular agents; Bioinformatics; Capital costs; Clinical isolates; Complexity; Computational biology; Consortia; Control; Cost-Benefit Analysis; Deoxyribonucleic acid; Diagnostic reagents; DNA; DNA sequencing; DNA, Bacterial - analysis; Drug resistance; Drug Resistance, Multiple, Bacterial - genetics; Evaluation; Genetic aspects; Genome-wide association studies; Genomes; Genomic libraries; Genomics; Health surveillance; High-Throughput Nucleotide Sequencing - economics; High-Throughput Nucleotide Sequencing - instrumentation; High-Throughput Nucleotide Sequencing - methods; High-throughput screening (Biochemical assaying); Humans; Infectious diseases; Instrumentation; Laboratories; Maintenance costs; Medical research; Medicine; Microbial drug resistance; Mortality; Mutation; Mycobacterium tuberculosis; Mycobacterium tuberculosis - genetics; Next-generation sequencing; Occupational health; Optimization; Patients; Public health; Reproducibility of Results; Risk factors; Sequence Analysis, DNA - economics; Sequence Analysis, DNA - instrumentation; Sequence Analysis, DNA - methods; Single-nucleotide polymorphism; Time Factors; Tuberculosis; Tuberculosis, Multidrug-Resistant - microbiology; Visualization; Whole genome sequencing; Workflow; Workflow software; California; United States > US; Switzerland; Arizona

Accurate, comprehensive, and timely detection of drug-resistant tuberculosis (TB) is essential to inform patient treatment and enable public health surveillance. This is crucial for effective control of TB globally. Whole-genome sequencing (WGS) and targeted next-generation sequencing (NGS) approach...