Development of a rapid cell‐fusion‐based phenotypic HIV‐1 tropism assay

• Published in: Journal of the International AIDS Society Vol. 16; no. 1; pp. 18723 - n/a
• Main Authors: Teeranaipong, Phairote, Hosoya, Noriaki, Kawana‐Tachikawa, Ai, Fujii, Takeshi, Koibuchi, Tomohiko, Nakamura, Hitomi, Koga, Michiko, Kondo, Naoyuki, Gao, George F, Hoshino, Hiroo, Matsuda, Zene, Iwamoto, Aikichi
• Format: Journal Article
• Language: English
• Published: Switzerland International AIDS Society 01.01.2013; John Wiley & Sons, Inc
• Subjects: Acquired immune deficiency syndrome; AIDS; Amino acids; Analysis; Biological assay; CD4 Antigens - genetics; Cell Fusion; Cell hybridization; Cell Line; chemokine receptor; Chemokines; Cloning; Construction contracts; co‐receptor; co‐receptor usage; Deoxyribonucleic acid; Diagnosis; DNA; Education; Experiments; fusion; Gene Expression; Genes, Reporter; Glycoproteins; Green Fluorescent Proteins - analysis; Green Fluorescent Proteins - genetics; HIV; HIV (Viruses); HIV infection; HIV Infections - virology; HIV-1 - isolation & purification; HIV-1 - physiology; HIV‐1; Human immunodeficiency virus; Humans; Identification and classification; Immune system; Infections; Infectious diseases; Laboratories; Ligands; Localization; Luciferases - analysis; Luciferases - genetics; Methods; Penicillin; Pharmaceuticals; Phenotype; Plasmids; Product development; Proteins; Receptors, CCR5 - genetics; Receptors, CXCR4 - genetics; Receptors, HIV - genetics; Risk factors; Science; tropism; Tropisms; Viral Tropism; Virology - methods; Japan; United States > US; HIV-1; fusion; tropism; co-receptor; co-receptor usage; chemokine receptor

Introduction A dual split reporter protein system (DSP), recombining Renilla luciferase (RL) and green fluorescent protein (GFP) split into two different constructs (DSP1–7 and DSP8–11), was adapted to create a novel rapid phenotypic tropism assay (PTA) for HIV‐1 infection (DSP‐Pheno). Methods DSP1–...