Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

• Published in: Molecular plant pathology Vol. 17; no. 7; pp. 1140 - 1153
• Main Authors: Chandrasekaran, Jeyabharathy, Brumin, Marina, Wolf, Dalia, Leibman, Diana, Klap, Chen, Pearlsman, Mali, Sherman, Amir, Arazi, Tzahi, Gal-On, Amit
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.09.2016; John Wiley & Sons, Inc; John Wiley and Sons Inc
• Subjects: backcrossing; Base Sequence; Chromosome Segregation - genetics; CRISPR-Cas Systems - genetics; CRISPR/Cas9; crops; cucumber; Cucumber vein yellowing virus; cucumbers; Cucumis sativus; Cucumis sativus - genetics; Cucumis sativus - virology; Disease Resistance - genetics; eIF4E; Eukaryotic Initiation Factor-4E - genetics; Gene Editing; Genes; Genome editing; Genotype; heterozygosity; homozygosity; Homozygote; immunity; mutants; Mutation - genetics; Original; Papaya mosaic virus; Papaya ringspot virus; plant development; Plant Diseases - virology; Plant Viruses - physiology; Plants, Genetically Modified; Potyviridae; progeny; RNA; single nucleotide polymorphism; technology; translation (genetics); Vegetables; virus resistance; Viruses; Zucchini yellow mosaic virus; genome editing; eIF4E; CRISPR/Cas9; cucumber; Potyviridae; virus resistance

Summary Genome editing in plants has been boosted tremendously by the development of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. This powerful tool allows substantial improvement in plant traits in addition to those provided by classical breeding. Here, we dem...