A general protocol for the crystallization of membrane proteins for X-ray structural investigation

• Published in: Nature protocols Vol. 4; no. 5; pp. 619 - 637
• Main Authors: Stroud, Robert M, Newby, Zachary E R, O'Connell, Joseph D, Gruswitz, Franz, Hays, Franklin A, Harries, William E C, Harwood, Ian M, Ho, Joseph D, Lee, John K, Savage, David F, Miercke, Larry J W
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2009; Nature Publishing Group
• Subjects: Analytical Chemistry; Biological Techniques; Biomedical and Life Sciences; Chromatography, Affinity; Chromatography, Gel; Cloning, Molecular; Computational Biology/Bioinformatics; Crystallization; Crystallization - methods; Crystallography; Crystallography, X-Ray; Crystals; Deoxyribonucleic acid; Detergents; DNA; Escherichia coli - genetics; Ions; Life Sciences; Membrane proteins; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - isolation & purification; Membranes; Methods; Microarrays; Organic Chemistry; Protein research; Protein Structure, Tertiary; Proteins; Protocol; Solubility; Structure; X-ray crystallography; X-ray diffraction; Yeast; United States; United States > US; San Francisco California; California

Protein crystallography is used to generate atomic resolution structures of protein molecules. These structures provide information about biological function, mechanism and interaction of a protein with substrates or effectors including DNA, RNA, cofactors or other small molecules, ions and other pr...