Distinct Peripheral Blood RNA Responses to Salmonella in Pigs Differing in Salmonella Shedding Levels: Intersection of IFNG, TLR and miRNA Pathways

• Published in: PloS one Vol. 6; no. 12; p. e28768
• Main Authors: Huang, Ting-Hua, Uthe, Jolita J, Bearson, Shawn M. D, Demirkale, Cumhur Yusuf, Nettleton, Dan, Knetter, Susan, Christian, Curtis, Ramer-Tait, Amanda E, Wannemuehler, Michael J, Tuggle, Christopher K, Deb, Sumitra
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.12.2011; Public Library of Science (PLoS)
• Subjects: Agriculture; Analysis; Animal diseases; Animal husbandry; Animal sciences; Animals; Area Under Curve; Bacteria; Bacterial Shedding - physiology; Bioindicators; Biology; Biomarkers; Blood; carrier state; Cluster Analysis; Colonization; Cytokines; Dendritic cells; Drugs; Feces; Foodborne diseases; foodborne illness; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Gene Regulatory Networks - genetics; Genes; Genetic aspects; Genetics; Hogs; Immunoglobulins; Inoculation; Interferon; interferon-gamma; Interferon-gamma - genetics; Interferon-gamma - metabolism; Medical research; Medicine; MicroRNA; MicroRNAs; MicroRNAs - blood; MicroRNAs - genetics; miRNA; Molecular Sequence Annotation; NF-κB protein; Nutrition; Oligonucleotide Array Sequence Analysis; pathogens; Peripheral blood; phenotype; Pigs; Polymerase Chain Reaction; Protein synthesis; Proteins; Reproducibility of Results; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Salmonella; Salmonella food poisoning; Salmonella Infections, Animal - blood; Salmonella Infections, Animal - genetics; Salmonella Infections, Animal - microbiology; Salmonella Typhimurium; Salmonella typhimurium - physiology; Shedding; Signal Transduction - genetics; Studies; Sus scrofa - blood; Sus scrofa - classification; Sus scrofa - genetics; Sus scrofa - microbiology; Swine; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism; transcription (genetics); transcription factor NF-kappa B; transcriptomics; tumor necrosis factors; variance; Veterinary colleges; Veterinary medicine; Veterinary Science; Zoology; Zoonoses; γ-Interferon; United States > US; Iowa

Transcriptomic analysis of the response to bacterial pathogens has been reported for several species, yet few studies have investigated the transcriptional differences in whole blood in subjects that differ in their disease response phenotypes. Salmonella species infect many vertebrate species, and...