Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells

• Published in: Nature biotechnology Vol. 31; no. 12; pp. 1126 - 1132
• Main Authors: Gole, Jeff, Gore, Athurva, Richards, Andrew, Chiu, Yu-Jui, Fung, Ho-Lim, Bushman, Diane, Chiang, Hsin-I, Chun, Jerold, Lo, Yu-Hwa, Zhang, Kun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2013; Nature Publishing Group
• Subjects: 631/61/514/2254; Agriculture; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Cell Separation - instrumentation; Cells; Chromosome Mapping - instrumentation; Cloning; Cloning, Molecular - methods; Deoxyribonucleic acid; DNA; DNA - genetics; DNA polymerases; DNA sequencing; DNA-Directed DNA Polymerase - genetics; E coli; Equipment Design; Equipment Failure Analysis; Gene amplification; Genetic aspects; Genomics; High-Throughput Nucleotide Sequencing - instrumentation; High-Throughput Nucleotide Sequencing - methods; Life Sciences; Mammals; Methods; Microorganisms; Mutation; Nanotechnology - instrumentation; Nanotechnology - methods; Nucleotide sequencing; Physiological aspects; Reactors; United States

Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution. Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying s...