Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells - Gole, Jeff; Gore, Athurva; Richards, Andrew; Chiu, Yu-Jui; Fung, Ho-Lim; Bushman, Diane; Chiang, Hsin-I; Chun, Jerold; Lo, Yu-Hwa; Zhang, Kun (Nature biotechnology, 2013)
Keywords: 631/61/514/2254; Agriculture; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Cell Separation - instrumentation; Cells; Chromosome Mapping - instrumentation; Cloning; Cloning, Molecular - methods; Deoxyribonucleic acid; DNA; DNA - genetics; DNA polymerases; DNA sequencing; DNA-Directed DNA Polymerase - genetics; E coli; Equipment Design; Equipment Failure Analysis; Gene amplification; Genetic aspects; Genomics; High-Throughput Nucleotide Sequencing - instrumentation; High-Throughput Nucleotide Sequencing - methods; Life Sciences; Mammals; Methods; Microorganisms; Mutation; Nanotechnology - instrumentation; Nanotechnology - methods; Nucleotide sequencing; Physiological aspects; Reactors; United States