Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells

Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution. Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying s...

Full description

Saved in:
Bibliographic Details
Published inNature biotechnology Vol. 31; no. 12; pp. 1126 - 1132
Main Authors Gole, Jeff, Gore, Athurva, Richards, Andrew, Chiu, Yu-Jui, Fung, Ho-Lim, Bushman, Diane, Chiang, Hsin-I, Chun, Jerold, Lo, Yu-Hwa, Zhang, Kun
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 01.12.2013
Nature Publishing Group
Subjects
631/61/514/2254
Agriculture
Bioinformatics
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cell Separation - instrumentation
Cells
Chromosome Mapping - instrumentation
Cloning
Cloning, Molecular - methods
Deoxyribonucleic acid
DNA
DNA - genetics
DNA polymerases
DNA sequencing
DNA-Directed DNA Polymerase - genetics
E coli
Equipment Design
Equipment Failure Analysis
Gene amplification
Genetic aspects
Genomics
High-Throughput Nucleotide Sequencing - instrumentation
High-Throughput Nucleotide Sequencing - methods
Life Sciences
Mammals
Methods
Microorganisms
Mutation
Nanotechnology - instrumentation
Nanotechnology - methods
Nucleotide sequencing
Physiological aspects
Reactors
United States
Online AccessGet full text

Cover

Abstract Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution. Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and their genetic material is simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated, nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single Escherichia coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1- to 2-Mb resolution. MIDAS can potentially further the characterization of genomic diversity in many heterogeneous cell populations.
AbstractList Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and their genetic material is simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated, nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single Escherichia coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1- to 2-Mb resolution. MIDAS can potentially further the characterization of genomic diversity in many heterogeneous cell populations.Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and their genetic material is simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated, nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single Escherichia coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1- to 2-Mb resolution. MIDAS can potentially further the characterization of genomic diversity in many heterogeneous cell populations.
Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution.
Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and their genetic material is simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated, nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single Escherichia coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1- to 2-Mb resolution. MIDAS can potentially further the characterization of genomic diversity in many heterogeneous cell populations.
Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution. Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and their genetic material is simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated, nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single Escherichia coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1- to 2-Mb resolution. MIDAS can potentially further the characterization of genomic diversity in many heterogeneous cell populations.
Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single E. coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1–2 Mb resolution. MIDAS will further the characterization of genomic diversity in many heterogeneous cell populations.
Audience Academic
Author Gore, Athurva
Richards, Andrew
Lo, Yu-Hwa
Chiang, Hsin-I
Chun, Jerold
Zhang, Kun
Gole, Jeff
Bushman, Diane
Fung, Ho-Lim
Chiu, Yu-Jui
AuthorAffiliation 1 Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA
2 Materials Science and Engineering Program, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA
3 Dorris Neuroscience Center, Molecular and Cellular Neuroscience Department, The Scripps Research Institute, La Jolla, California 92037
4 Department of Electrical and Computer Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA
AuthorAffiliation_xml – name: 1 Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA
– name: 2 Materials Science and Engineering Program, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA
– name: 3 Dorris Neuroscience Center, Molecular and Cellular Neuroscience Department, The Scripps Research Institute, La Jolla, California 92037
– name: 4 Department of Electrical and Computer Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA
Author_xml – sequence: 1
  givenname: Jeff
  surname: Gole
  fullname: Gole, Jeff
  organization: Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego
– sequence: 2
  givenname: Athurva
  surname: Gore
  fullname: Gore, Athurva
  organization: Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego
– sequence: 3
  givenname: Andrew
  surname: Richards
  fullname: Richards, Andrew
  organization: Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego
– sequence: 4
  givenname: Yu-Jui
  surname: Chiu
  fullname: Chiu, Yu-Jui
  organization: Materials Science and Engineering Program, University of California at San Diego
– sequence: 5
  givenname: Ho-Lim
  surname: Fung
  fullname: Fung, Ho-Lim
  organization: Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego
– sequence: 6
  givenname: Diane
  surname: Bushman
  fullname: Bushman, Diane
  organization: Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute
– sequence: 7
  givenname: Hsin-I
  surname: Chiang
  fullname: Chiang, Hsin-I
  organization: Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego, Present address: Department of Animal Science, National Chung Hsing University, Taichung, Taiwan
– sequence: 8
  givenname: Jerold
  surname: Chun
  fullname: Chun, Jerold
  organization: Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute
– sequence: 9
  givenname: Yu-Hwa
  surname: Lo
  fullname: Lo, Yu-Hwa
  organization: Department of Electrical and Computer Engineering, University of California at San Diego
– sequence: 10
  givenname: Kun
  surname: Zhang
  fullname: Zhang, Kun
  email: kzhang@bioeng.ucsd.edu
  organization: Department of Bioengineering, Institute for Genomic Medicine and Institute of Engineering in Medicine, University of California at San Diego
BackLink https://www.ncbi.nlm.nih.gov/pubmed/24213699$$D View this record in MEDLINE/PubMed
BookMark eNqNk9tq3DAQhk1JaQ4t9AmKoTct1FsdVpJ9Uwihh0BKoKdboZVHjoIsbS077b59JbLd7IbQLr6QJX3zSzP657g48MFDUTzHaIYRrd_6xTgjgqBHxRFmc15h3vCD9I9qUSHM-GFxHOM1QojPOX9SHJI5wZQ3zVFhPqsY7Q24VblUg3IOXLkMbtXDoCKU2gVvfVcq35Yd-NBDGeHnBF7n1WDKmEaXOHAullOelV754OwIQ9lbPYRfeetp8dgoF-HZejwpvn94_-3sU3Vx-fH87PSi0jViY9VQbRZaiAU2tSFgWqE4BtHMG1ZjUpO2YQy1DTWUNRq3BLWatC0gXhNoDNX0pHh3q7ucFj20GvyYkpLLwfZqWMmgrNzd8fZKduFG0lowiusk8GotMISUZxxlb2POTnkIU5Q4FZAKxlFGX95Dr8M0-JSexIwJjglNVd5QnXIgrTchnauzqDzlhFPaiDn6J0UZpYRyJBI1e4BKXwup1MkSxqb1nYDXOwGJGeH32KkpRnn-9cvuFf7H7qt7-WN_3cxu677ZYhfZT5Ds6aPtrsZ4G7IjvQe-rf5i2xwbV_zthrvHT7aNcQCzQTCSudFkajSZG-3uKTaotqMabci2su6hgHUZYtL0HQxbbrnP_gE6ujOi
CitedBy_id crossref_primary_10_1002_smll_202100325
crossref_primary_10_1126_sciadv_abd6454
crossref_primary_10_1186_s13059_016_0941_0
crossref_primary_10_1016_j_nbd_2023_106208
crossref_primary_10_1007_s00604_016_1745_3
crossref_primary_10_1038_s41598_022_20923_z
crossref_primary_10_1016_j_tig_2015_03_013
crossref_primary_10_1093_bib_bbad275
crossref_primary_10_4109_jslab_31_17
crossref_primary_10_1073_pnas_1520964113
crossref_primary_10_1002_pmic_201700312
crossref_primary_10_3390_genes12071071
crossref_primary_10_1039_D2LC00888B
crossref_primary_10_1002_dneu_22626
crossref_primary_10_1016_j_aca_2024_342217
crossref_primary_10_1016_j_coisb_2017_05_001
crossref_primary_10_1021_acs_analchem_9b05345
crossref_primary_10_1016_j_crmeth_2021_100054
crossref_primary_10_1111_dgd_12898
crossref_primary_10_1016_j_snb_2024_135391
crossref_primary_10_1038_nmeth_2768
crossref_primary_10_1016_j_canlet_2015_04_022
crossref_primary_10_3390_ijms21051860
crossref_primary_10_1002_chem_201800305
crossref_primary_10_3389_fncel_2022_918041
crossref_primary_10_1016_j_cell_2015_10_039
crossref_primary_10_1101_gr_223263_117
crossref_primary_10_3390_ijms19092731
crossref_primary_10_1038_nbt_4038
crossref_primary_10_1093_brain_awy157
crossref_primary_10_1186_s40364_024_00654_1
crossref_primary_10_1038_nbt_2892
crossref_primary_10_1038_nrg_2017_15
crossref_primary_10_1039_C5RA09056C
crossref_primary_10_1021_ja5053279
crossref_primary_10_1093_bioinformatics_btu540
crossref_primary_10_1038_s42003_024_06940_w
crossref_primary_10_1371_journal_pone_0138733
crossref_primary_10_1101_gr_168286_113
crossref_primary_10_1126_science_aaf1204
crossref_primary_10_1002_admt_202401018
crossref_primary_10_1021_acs_analchem_7b02183
crossref_primary_10_1038_s41467_023_41019_w
crossref_primary_10_1038_s41598_017_04426_w
crossref_primary_10_1016_j_nantod_2023_101807
crossref_primary_10_1038_nrc_2017_58
crossref_primary_10_1039_C7LC00924K
crossref_primary_10_1002_adhm_202302011
crossref_primary_10_1038_ncomms7822
crossref_primary_10_1099_mgen_0_000871
crossref_primary_10_3390_genes9010050
crossref_primary_10_1016_j_actbio_2021_03_006
crossref_primary_10_1093_gbe_evx256
crossref_primary_10_1039_C8NR04917C
crossref_primary_10_1116_1_5037582
crossref_primary_10_1021_acs_analchem_8b00362
crossref_primary_10_1038_srep11415
crossref_primary_10_1039_C7LC01288H
crossref_primary_10_1146_annurev_bioeng_060418_052538
crossref_primary_10_1016_j_cell_2015_03_026
crossref_primary_10_1039_C6LC01313A
crossref_primary_10_1039_C4LC00013G
crossref_primary_10_1016_j_celrep_2014_07_043
crossref_primary_10_1016_j_gde_2014_06_004
crossref_primary_10_1007_s10404_021_02485_0
crossref_primary_10_1016_j_mrfmmm_2018_06_002
crossref_primary_10_1186_s12943_021_01392_w
crossref_primary_10_1039_C9AY01922G
crossref_primary_10_1039_C6RA21229H
crossref_primary_10_1246_cl_210066
crossref_primary_10_1097_POC_0000000000000052
crossref_primary_10_1177_0022034520907380
crossref_primary_10_1016_j_trac_2017_11_017
crossref_primary_10_1109_TCAD_2018_2883908
crossref_primary_10_1016_j_csbj_2023_02_034
crossref_primary_10_1038_nrg3785
crossref_primary_10_1093_hmg_ddz191
crossref_primary_10_1093_nar_gkv1493
crossref_primary_10_1016_j_mee_2017_11_011
crossref_primary_10_1038_nbt_3880
crossref_primary_10_1016_j_gde_2013_12_004
crossref_primary_10_3390_ijms18030492
crossref_primary_10_1111_bpa_12958
crossref_primary_10_1002_ange_201807314
crossref_primary_10_1038_nrg_2015_16
crossref_primary_10_1371_journal_pone_0135007
crossref_primary_10_1016_j_molcel_2015_05_005
crossref_primary_10_1016_j_neuron_2016_02_004
crossref_primary_10_1371_journal_pgen_1004126
crossref_primary_10_1007_s10577_023_09740_w
crossref_primary_10_1016_j_mtbio_2022_100222
crossref_primary_10_1038_nbt_3711
crossref_primary_10_1039_C6AN00164E
crossref_primary_10_1126_science_aab1601
crossref_primary_10_1186_1471_2164_15_443
crossref_primary_10_1002_humu_22625
crossref_primary_10_1111_nan_12465
crossref_primary_10_1002_mds_25883
crossref_primary_10_1038_s42003_019_0401_y
crossref_primary_10_1074_jbc_REV120_009192
crossref_primary_10_1021_acs_analchem_0c00037
crossref_primary_10_1007_s00253_020_10844_0
crossref_primary_10_1111_cas_14821
crossref_primary_10_1186_s13742_015_0091_4
crossref_primary_10_1038_s43705_022_00179_4
crossref_primary_10_1073_pnas_1812702115
crossref_primary_10_1002_dro2_74
crossref_primary_10_1039_C8LC00169C
crossref_primary_10_1128_mSystems_00181_21
crossref_primary_10_3390_mi11070645
crossref_primary_10_1002_smll_202402177
crossref_primary_10_1109_TCAD_2019_2948907
crossref_primary_10_1016_j_trac_2022_116894
crossref_primary_10_1080_07388551_2018_1427697
crossref_primary_10_1038_s41598_018_20384_3
crossref_primary_10_3390_bioengineering9110674
crossref_primary_10_1038_nmeth_4469
crossref_primary_10_1016_j_coisb_2017_11_008
crossref_primary_10_1093_molehr_gav050
crossref_primary_10_1186_s12864_016_2480_1
crossref_primary_10_3390_bios12020058
crossref_primary_10_1039_C6LC01241H
crossref_primary_10_1063_1_5131795
crossref_primary_10_1039_D2LC00667G
crossref_primary_10_3389_fimmu_2023_1276194
crossref_primary_10_1146_annurev_genet_120213_092412
crossref_primary_10_1016_j_jaut_2022_102919
crossref_primary_10_1186_s13059_018_1593_z
crossref_primary_10_1039_C7AN01346A
crossref_primary_10_1093_hmg_ddv235
crossref_primary_10_1186_s40478_019_0873_5
crossref_primary_10_1371_journal_pone_0191520
crossref_primary_10_1007_s11434_014_0634_6
crossref_primary_10_3389_fpls_2019_01170
crossref_primary_10_1016_j_coche_2014_01_011
crossref_primary_10_1111_nyas_13296
crossref_primary_10_7554_eLife_05116
crossref_primary_10_1007_s40484_020_0217_2
crossref_primary_10_1002_adbi_201900188
crossref_primary_10_1039_C7LC00430C
crossref_primary_10_3389_fmicb_2014_00771
crossref_primary_10_1186_s12896_017_0408_8
crossref_primary_10_1002_smll_201903905
crossref_primary_10_1007_s00412_016_0615_4
crossref_primary_10_1016_j_medj_2022_09_009
crossref_primary_10_1002_anie_201807314
crossref_primary_10_3390_microorganisms3020236
crossref_primary_10_1186_s13059_015_0616_2
crossref_primary_10_1126_science_aak9787
crossref_primary_10_1371_journal_pone_0137677
crossref_primary_10_1073_pnas_1513988112
crossref_primary_10_1021_acssensors_7b00873
crossref_primary_10_1002_0471142727_mb0721s112
crossref_primary_10_1038_s41593_018_0257_3
crossref_primary_10_1371_journal_pone_0105585
crossref_primary_10_1158_0008_5472_CAN_15_1907
crossref_primary_10_1016_j_jgg_2014_09_005
crossref_primary_10_1186_s13100_018_0128_1
crossref_primary_10_1016_j_copbio_2014_10_010
crossref_primary_10_1021_acs_chemrev_5b00428
crossref_primary_10_3390_ijms24054270
crossref_primary_10_1371_journal_pone_0153699
crossref_primary_10_1016_j_biocel_2017_09_018
crossref_primary_10_1038_s41598_017_05436_4
crossref_primary_10_1101_gr_262667_120
Cites_doi 10.1038/nature09871
10.1371/journal.pone.0010314
10.1038/nprot.2012.039
10.1093/nar/gkq1074
10.1523/JNEUROSCI.4560-04.2005
10.1126/science.1203163
10.1016/j.cell.2012.02.025
10.1093/nar/gkm321
10.1002/cne.22436
10.1073/pnas.0508809102
10.1016/j.cell.2012.02.028
10.1093/bioinformatics/btt086
10.1093/nar/gkl350
10.1126/science.1229164
10.1186/1471-2164-9-75
10.1038/nature09807
10.1101/gr.144600.112
10.1038/nbt.1739
10.1073/pnas.0704662104
10.1126/science.1229112
10.1073/pnas.231487398
10.1016/j.tins.2010.04.001
10.1126/science.1117389
10.1371/journal.pone.0006864
10.1523/JNEUROSCI.23-32-10454.2003
10.1101/gr.5969107
10.1371/journal.pone.0000558
10.1101/gr.136242.111
10.1016/j.cell.2012.06.030
10.1002/cne.21648
10.1038/nature04959
10.1371/journal.pgen.0030155
10.1038/nature11632
10.1089/cmb.2012.0021
10.1073/pnas.0808028105
10.1038/nbt.1966
10.1101/gr.142208.112
10.1038/nbt1214
10.1038/nbt.2579
ContentType Journal Article
Copyright Springer Nature Limited 2013
COPYRIGHT 2013 Nature Publishing Group
Copyright Nature Publishing Group Dec 2013
Copyright_xml – notice: Springer Nature Limited 2013
– notice: COPYRIGHT 2013 Nature Publishing Group
– notice: Copyright Nature Publishing Group Dec 2013
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
N95
IOV
ISR
3V.
7QO
7QP
7QR
7T7
7TK
7TM
7X7
7XB
88A
88E
88I
8AO
8FD
8FE
8FG
8FH
8FI
8FJ
8FK
8G5
ABJCF
ABUWG
AEUYN
AFKRA
AZQEC
BBNVY
BENPR
BGLVJ
BHPHI
C1K
CCPQU
DWQXO
FR3
FYUFA
GHDGH
GNUQQ
GUQSH
HCIFZ
K9.
L6V
LK8
M0S
M1P
M2O
M2P
M7P
M7S
MBDVC
P64
PHGZM
PHGZT
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PTHSS
Q9U
RC3
7X8
5PM
DOI 10.1038/nbt.2720
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Gale Business: Insights
Gale In Context: Opposing Viewpoints
Gale In Context: Science
ProQuest Central (Corporate)
Biotechnology Research Abstracts
Calcium & Calcified Tissue Abstracts
Chemoreception Abstracts
Industrial and Applied Microbiology Abstracts (Microbiology A)
Neurosciences Abstracts
Nucleic Acids Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Biology Database (Alumni Edition)
Medical Database (Alumni Edition)
Science Database (Alumni Edition)
ProQuest Pharma Collection
Technology Research Database
ProQuest SciTech Collection
ProQuest Technology Collection
ProQuest Natural Science Collection
ProQuest Hospital Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Research Library
Materials Science & Engineering Collection
ProQuest Central (Alumni)
ProQuest One Sustainability (subscription)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Database
ProQuest Central
Technology Collection
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One Community College
ProQuest Central
Engineering Research Database
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
Research Library Prep
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
ProQuest Engineering Collection
Biological Sciences
ProQuest Health & Medical Collection
PML(ProQuest Medical Library)
Research Library
Science Database
Biological Science Database
Engineering Database
Research Library (Corporate)
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
Engineering collection
ProQuest Central Basic
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Research Library Prep
ProQuest Central Student
ProQuest Central Essentials
Nucleic Acids Abstracts
SciTech Premium Collection
Environmental Sciences and Pollution Management
ProQuest One Applied & Life Sciences
ProQuest One Sustainability
Health Research Premium Collection
Natural Science Collection
Health & Medical Research Collection
Biological Science Collection
Chemoreception Abstracts
Industrial and Applied Microbiology Abstracts (Microbiology A)
ProQuest Central (New)
ProQuest Medical Library (Alumni)
Engineering Collection
Engineering Database
ProQuest Science Journals (Alumni Edition)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
ProQuest Technology Collection
Health Research Premium Collection (Alumni)
Biological Science Database
Neurosciences Abstracts
ProQuest Hospital Collection (Alumni)
Biotechnology and BioEngineering Abstracts
ProQuest Health & Medical Complete
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
Calcium & Calcified Tissue Abstracts
ProQuest One Academic (New)
Technology Collection
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest One Health & Nursing
Research Library (Alumni Edition)
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Biology Journals (Alumni Edition)
ProQuest Central
ProQuest Health & Medical Research Collection
Genetics Abstracts
ProQuest Engineering Collection
Biotechnology Research Abstracts
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
ProQuest Research Library
ProQuest Central Basic
ProQuest Science Journals
ProQuest SciTech Collection
ProQuest Medical Library
Materials Science & Engineering Collection
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic



MEDLINE
Research Library Prep
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: 8FG
  name: ProQuest Technology Collection
  url: https://search.proquest.com/technologycollection1
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Engineering
Agriculture
Biology
EISSN 1546-1696
EndPage 1132
ExternalDocumentID PMC3875318
3415707621
A626339740
A353323607
24213699
10_1038_nbt_2720
Genre Research Support, U.S. Gov't, Non-P.H.S
Journal Article
Research Support, N.I.H., Extramural
Feature
GeographicLocations United States
GeographicLocations_xml – name: United States
GrantInformation_xml – fundername: NHGRI NIH HHS
  grantid: P50 HG005550
– fundername: NHGRI NIH HHS
  grantid: R01HG004876
– fundername: NIGMS NIH HHS
  grantid: T32 GM007752
– fundername: NIGMS NIH HHS
  grantid: R01 GM097253
– fundername: NIMH NIH HHS
  grantid: U01 MH098977
– fundername: NHGRI NIH HHS
  grantid: R01 HG004876
– fundername: NHGRI NIH HHS
  grantid: P50HG005550
– fundername: NIMH NIH HHS
  grantid: U01MH098977
– fundername: NIGMS NIH HHS
  grantid: R01GM097253
GroupedDBID ---
-~X
.55
.GJ
0R~
123
29M
2FS
2XV
36B
39C
3V.
4.4
4R4
53G
5BI
5M7
5RE
5S5
70F
7X7
88A
88E
88I
8AO
8CJ
8FE
8FG
8FH
8FI
8FJ
8G5
8R4
8R5
A8Z
AAEEF
AAHBH
AAIKC
AAMNW
AARCD
AAYOK
AAYZH
AAZLF
ABAWZ
ABDBF
ABDPE
ABEFU
ABJCF
ABJNI
ABLJU
ABOCM
ABUWG
ACBTR
ACBWK
ACGFO
ACGFS
ACGOD
ACIWK
ACMJI
ACPRK
ACUHS
ADBBV
ADFRT
AENEX
AEUYN
AFANA
AFBBN
AFFNX
AFKRA
AFRAH
AFSHS
AGAYW
AGHTU
AHBCP
AHMBA
AHOSX
AHSBF
AIBTJ
ALFFA
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMTXH
ARMCB
ASPBG
AVWKF
AXYYD
AZFZN
AZQEC
BAAKF
BBNVY
BENPR
BGLVJ
BHPHI
BKKNO
BKOMP
BPHCQ
BVXVI
C0K
CCPQU
D1J
DB5
DU5
DWQXO
EAD
EAP
EAS
EBC
EBS
EE.
EJD
EMB
EMK
EMOBN
ESX
EXGXG
F5P
FA8
FEDTE
FQGFK
FSGXE
FYUFA
GNUQQ
GUQSH
GX1
HCIFZ
HMCUK
HVGLF
HZ~
IAG
IAO
IEA
IEP
IH2
IHR
INH
INR
IOV
ISR
ITC
KOO
L6V
LGEZI
LK8
LOTEE
M0L
M1P
M2O
M2P
M7P
M7S
ML0
MVM
N95
NADUK
NEJ
NNMJJ
NXXTH
O9-
ODYON
P2P
PKN
PQQKQ
PROAC
PSQYO
PTHSS
Q2X
QF4
QM4
QN7
QO4
RNS
RNT
RNTTT
RVV
RXW
SHXYY
SIXXV
SJN
SNYQT
SOJ
SV3
TAE
TAOOD
TBHMF
TDRGL
TN5
TSG
TUS
U5U
UKHRP
X7M
XI7
XOL
Y6R
YZZ
ZGI
ZHY
ZXP
~KM
AAYXX
ACMFV
ACSTC
ALPWD
ATHPR
CITATION
PHGZM
PHGZT
CGR
CUY
CVF
ECM
EIF
NPM
PMFND
7QO
7QP
7QR
7T7
7TK
7TM
7XB
8FD
8FK
C1K
FR3
K9.
MBDVC
P64
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQUKI
Q9U
RC3
7X8
5PM
ID FETCH-LOGICAL-c805t-93cfbc77b1f8f2efd7a61e7949581282d9550d93f359c1d20dc2dde0682e9f3c3
IEDL.DBID 7X7
ISSN 1087-0156
1546-1696
IngestDate Thu Aug 21 14:12:31 EDT 2025
Tue Aug 05 11:27:56 EDT 2025
Fri Jul 25 08:53:08 EDT 2025
Tue Jun 17 21:25:28 EDT 2025
Tue Jun 17 21:17:46 EDT 2025
Tue Jun 10 20:33:24 EDT 2025
Fri Jun 27 05:04:43 EDT 2025
Fri Jun 27 04:55:38 EDT 2025
Fri Jun 27 03:59:00 EDT 2025
Fri Jun 27 04:22:57 EDT 2025
Fri Jun 27 02:53:16 EDT 2025
Fri Jun 27 03:11:29 EDT 2025
Wed Feb 19 02:42:44 EST 2025
Thu Apr 24 23:11:38 EDT 2025
Tue Jul 01 04:29:34 EDT 2025
Fri Feb 21 02:39:45 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 12
Language English
License http://www.springer.com/tdm
Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c805t-93cfbc77b1f8f2efd7a61e7949581282d9550d93f359c1d20dc2dde0682e9f3c3
Notes SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ObjectType-Article-1
ObjectType-Feature-2
content type line 23
Present address: Department of Animal Science, National Chung Hsing University, Taichung, Taiwan
OpenAccessLink https://pubmed.ncbi.nlm.nih.gov/PMC3875318
PMID 24213699
PQID 1557612321
PQPubID 47191
PageCount 7
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_3875318
proquest_miscellaneous_1466375608
proquest_journals_1557612321
gale_infotracmisc_A626339740
gale_infotracmisc_A353323607
gale_infotracacademiconefile_A353323607
gale_incontextgauss_ISR_A626339740
gale_incontextgauss_ISR_A353323607
gale_incontextgauss_IOV_A626339740
gale_incontextgauss_IOV_A353323607
gale_businessinsightsgauss_A626339740
gale_businessinsightsgauss_A353323607
pubmed_primary_24213699
crossref_primary_10_1038_nbt_2720
crossref_citationtrail_10_1038_nbt_2720
springer_journals_10_1038_nbt_2720
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2013-12-01
PublicationDateYYYYMMDD 2013-12-01
PublicationDate_xml – month: 12
  year: 2013
  text: 2013-12-01
  day: 01
PublicationDecade 2010
PublicationPlace London
PublicationPlace_xml – name: London
– name: United States
– name: New York
PublicationSubtitle The Science and Business of Biotechnology
PublicationTitle Nature biotechnology
PublicationTitleAbbrev Nat Biotechnol
PublicationTitleAlternate Nat Biotechnol
PublicationYear 2013
Publisher Nature Publishing Group UK
Nature Publishing Group
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Publishing Group
References Adey, Shendure (CR20) 2012; 22
Hou (CR4) 2012; 148
Yurov (CR24) 2007; 2
Marcy (CR6) 2007; 104
Moriya, Itoh, Okuda, Yoshizawa, Kanehisa (CR39) 2007; 35
Fitzsimons (CR17) 2013; 23
Singer, McConnell, Marchetto, Coufal, Gage (CR26) 2010; 33
Baslan (CR28) 2012; 7
Kirkness (CR32) 2013; 23
Albertsen (CR31) 2013; 31
Hutchison, Smith, Pfannkoch, Venter (CR13) 2005; 102
Yang (CR23) 2003; 23
Wang, Fan, Behr, Quake (CR10) 2012; 150
Xu (CR9) 2012; 148
Inoue, Shigemori, Mikawa (CR15) 2006; 34
Hussein (CR34) 2011; 471
Zhang (CR1) 2006; 24
Rehen (CR22) 2001; 98
Rodrigue (CR2) 2009; 4
Marcy (CR14) 2007; 3
Bankevich (CR11) 2012; 19
Blainey, Quake (CR19) 2011; 39
Gurevich, Saveliev, Vyahhi, Tesler (CR37) 2013; 29
Aziz (CR38) 2008; 9
Muotri, Gage (CR25) 2006; 441
Lu (CR33) 2012; 338
Woyke (CR16) 2010; 5
Fan, Wang, Potanina, Quake (CR3) 2011; 29
Huson, Auch, Qi, Schuster (CR36) 2007; 17
Navin (CR8) 2011; 472
Chitsaz (CR12) 2011; 29
Zong, Lu, Chapman, Xie (CR18) 2012; 338
Pan (CR5) 2008; 105
Shendure (CR29) 2005; 309
Abecasis (CR30) 2012; 491
Westra (CR27) 2010; 518
Rehen (CR21) 2005; 25
Yoon (CR7) 2011; 332
Westra (CR35) 2008; 507
MS Fitzsimons (BFnbt2720_CR17) 2013; 23
SM Hussein (BFnbt2720_CR34) 2011; 471
Y Marcy (BFnbt2720_CR14) 2007; 3
CA Hutchison III (BFnbt2720_CR13) 2005; 102
AH Yang (BFnbt2720_CR23) 2003; 23
T Woyke (BFnbt2720_CR16) 2010; 5
X Pan (BFnbt2720_CR5) 2008; 105
J Wang (BFnbt2720_CR10) 2012; 150
Y Moriya (BFnbt2720_CR39) 2007; 35
JW Westra (BFnbt2720_CR35) 2008; 507
JW Westra (BFnbt2720_CR27) 2010; 518
S Rodrigue (BFnbt2720_CR2) 2009; 4
A Gurevich (BFnbt2720_CR37) 2013; 29
PC Blainey (BFnbt2720_CR19) 2011; 39
SK Rehen (BFnbt2720_CR21) 2005; 25
X Xu (BFnbt2720_CR9) 2012; 148
K Zhang (BFnbt2720_CR1) 2006; 24
Y Marcy (BFnbt2720_CR6) 2007; 104
EF Kirkness (BFnbt2720_CR32) 2013; 23
S Lu (BFnbt2720_CR33) 2012; 338
M Albertsen (BFnbt2720_CR31) 2013; 31
SK Rehen (BFnbt2720_CR22) 2001; 98
H Chitsaz (BFnbt2720_CR12) 2011; 29
GR Abecasis (BFnbt2720_CR30) 2012; 491
DH Huson (BFnbt2720_CR36) 2007; 17
J Inoue (BFnbt2720_CR15) 2006; 34
A Adey (BFnbt2720_CR20) 2012; 22
A Bankevich (BFnbt2720_CR11) 2012; 19
C Zong (BFnbt2720_CR18) 2012; 338
HC Fan (BFnbt2720_CR3) 2011; 29
HS Yoon (BFnbt2720_CR7) 2011; 332
N Navin (BFnbt2720_CR8) 2011; 472
Y Hou (BFnbt2720_CR4) 2012; 148
AR Muotri (BFnbt2720_CR25) 2006; 441
T Singer (BFnbt2720_CR26) 2010; 33
RK Aziz (BFnbt2720_CR38) 2008; 9
YB Yurov (BFnbt2720_CR24) 2007; 2
J Shendure (BFnbt2720_CR29) 2005; 309
T Baslan (BFnbt2720_CR28) 2012; 7
11698687 - Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13361-6
15745943 - J Neurosci. 2005 Mar 2;25(9):2176-80
17593959 - PLoS One. 2007;2(6):e558
18273885 - J Comp Neurol. 2008 Apr 20;507(6):1944-51
21551060 - Science. 2011 May 6;332(6030):714-7
20737596 - J Comp Neurol. 2010 Oct 1;518(19):3981-4000
22385958 - Cell. 2012 Mar 2;148(5):886-95
23493677 - Genome Res. 2013 May;23(5):878-88
16732271 - Nat Biotechnol. 2006 Jun;24(6):680-6
21170043 - Nat Biotechnol. 2011 Jan;29(1):51-7
23707974 - Nat Biotechnol. 2013 Jun;31(6):533-8
22385957 - Cell. 2012 Mar 2;148(5):873-85
23422339 - Bioinformatics. 2013 Apr 15;29(8):1072-5
17255551 - Genome Res. 2007 Mar;17(3):377-86
23128226 - Nature. 2012 Nov 1;491(7422):56-65
22506599 - J Comput Biol. 2012 May;19(5):455-77
21926975 - Nat Biotechnol. 2011 Oct;29(10):915-21
21368824 - Nature. 2011 Mar 3;471(7336):58-62
18261238 - BMC Genomics. 2008;9:75
22817899 - Cell. 2012 Jul 20;150(2):402-12
18832167 - Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15499-504
14614104 - J Neurosci. 2003 Nov 12;23(32):10454-62
20471112 - Trends Neurosci. 2010 Aug;33(8):345-54
23282328 - Genome Res. 2013 May;23(5):826-32
19724646 - PLoS One. 2009;4(9):e6864
23258894 - Science. 2012 Dec 21;338(6114):1622-6
16286637 - Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17332-6
21399628 - Nature. 2011 Apr 7;472(7341):90-4
17526522 - Nucleic Acids Res. 2007 Jul;35(Web Server issue):W182-5
20428247 - PLoS One. 2010;5(4):e10314
21071419 - Nucleic Acids Res. 2011 Mar;39(4):e19
16810244 - Nature. 2006 Jun 29;441(7097):1087-93
22555242 - Nat Protoc. 2012 Jun;7(6):1024-41
23258895 - Science. 2012 Dec 21;338(6114):1627-30
16707659 - Nucleic Acids Res. 2006;34(9):e69
17620602 - Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11889-94
22466172 - Genome Res. 2012 Jun;22(6):1139-43
17892324 - PLoS Genet. 2007 Sep;3(9):1702-8
16081699 - Science. 2005 Sep 9;309(5741):1728-32
References_xml – volume: 471
  start-page: 58
  year: 2011
  end-page: 62
  ident: CR34
  article-title: Copy number variation and selection during reprogramming to pluripotency
  publication-title: Nature
  doi: 10.1038/nature09871
– volume: 5
  start-page: e10314
  year: 2010
  ident: CR16
  article-title: One bacterial cell, one complete genome
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0010314
– volume: 7
  start-page: 1024
  year: 2012
  end-page: 1041
  ident: CR28
  article-title: Genome-wide copy number analysis of single cells
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2012.039
– volume: 39
  start-page: e19
  year: 2011
  ident: CR19
  article-title: Digital MDA for enumeration of total nucleic acid contamination
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkq1074
– volume: 25
  start-page: 2176
  year: 2005
  end-page: 2180
  ident: CR21
  article-title: Constitutional aneuploidy in the normal human brain
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.4560-04.2005
– volume: 332
  start-page: 714
  year: 2011
  end-page: 717
  ident: CR7
  article-title: Single-cell genomics reveals organismal interactions in uncultivated marine protists
  publication-title: Science
  doi: 10.1126/science.1203163
– volume: 148
  start-page: 886
  year: 2012
  end-page: 895
  ident: CR9
  article-title: Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor
  publication-title: Cell
  doi: 10.1016/j.cell.2012.02.025
– volume: 35
  start-page: W182
  year: 2007
  end-page: W185
  ident: CR39
  article-title: KAAS: an automatic genome annotation and pathway reconstruction server
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkm321
– volume: 518
  start-page: 3981
  year: 2010
  end-page: 4000
  ident: CR27
  article-title: Neuronal DNA content variation (DCV) with regional and individual differences in the human brain
  publication-title: J. Comp. Neurol.
  doi: 10.1002/cne.22436
– volume: 102
  start-page: 17332
  year: 2005
  end-page: 17336
  ident: CR13
  article-title: Cell-free cloning using phi29 DNA polymerase
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0508809102
– volume: 148
  start-page: 873
  year: 2012
  end-page: 885
  ident: CR4
  article-title: Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm
  publication-title: Cell
  doi: 10.1016/j.cell.2012.02.028
– volume: 29
  start-page: 1072
  year: 2013
  end-page: 1075
  ident: CR37
  article-title: QUAST: quality assessment tool for genome assemblies
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btt086
– volume: 34
  start-page: e69
  year: 2006
  ident: CR15
  article-title: Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkl350
– volume: 338
  start-page: 1622
  year: 2012
  end-page: 1626
  ident: CR18
  article-title: Genome-wide detection of single-nucleotide and copy-number variations of a single human cell
  publication-title: Science
  doi: 10.1126/science.1229164
– volume: 9
  start-page: 75
  year: 2008
  ident: CR38
  article-title: The RAST Server: rapid annotations using subsystems technology
  publication-title: BMC Genomics
  doi: 10.1186/1471-2164-9-75
– volume: 472
  start-page: 90
  year: 2011
  end-page: 94
  ident: CR8
  article-title: Tumour evolution inferred by single-cell sequencing
  publication-title: Nature
  doi: 10.1038/nature09807
– volume: 23
  start-page: 826
  year: 2013
  end-page: 832
  ident: CR32
  article-title: Sequencing of isolated sperm cells for direct haplotyping of a human genome
  publication-title: Genome Res.
  doi: 10.1101/gr.144600.112
– volume: 29
  start-page: 51
  year: 2011
  end-page: 57
  ident: CR3
  article-title: Whole-genome molecular haplotyping of single cells
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1739
– volume: 104
  start-page: 11889
  year: 2007
  end-page: 11894
  ident: CR6
  article-title: Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0704662104
– volume: 338
  start-page: 1627
  year: 2012
  end-page: 1630
  ident: CR33
  article-title: Probing meiotic recombination and aneuploidy of single sperm cells by whole-genome sequencing
  publication-title: Science
  doi: 10.1126/science.1229112
– volume: 98
  start-page: 13361
  year: 2001
  end-page: 13366
  ident: CR22
  article-title: Chromosomal variation in neurons of the developing and adult mammalian nervous system
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.231487398
– volume: 33
  start-page: 345
  year: 2010
  end-page: 354
  ident: CR26
  article-title: LINE-1 retrotransposons: mediators of somatic variation in neuronal genomes?
  publication-title: Trends Neurosci.
  doi: 10.1016/j.tins.2010.04.001
– volume: 309
  start-page: 1728
  year: 2005
  end-page: 1732
  ident: CR29
  article-title: Accurate multiplex polony sequencing of an evolved bacterial genome
  publication-title: Science
  doi: 10.1126/science.1117389
– volume: 4
  start-page: e6864
  year: 2009
  ident: CR2
  article-title: Whole genome amplification and assembly of single bacterial cells
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0006864
– volume: 23
  start-page: 10454
  year: 2003
  end-page: 10462
  ident: CR23
  article-title: Chromosome segregation defects contribute to aneuploidy in normal neural progenitor cells
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.23-32-10454.2003
– volume: 17
  start-page: 377
  year: 2007
  end-page: 386
  ident: CR36
  article-title: MEGAN analysis of metagenomic data
  publication-title: Genome Res.
  doi: 10.1101/gr.5969107
– volume: 2
  start-page: e558
  year: 2007
  ident: CR24
  article-title: Aneuploidy and confined chromosomal mosaicism in the developing human brain
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0000558
– volume: 22
  start-page: 1139
  year: 2012
  end-page: 1143
  ident: CR20
  article-title: Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing
  publication-title: Genome Res.
  doi: 10.1101/gr.136242.111
– volume: 150
  start-page: 402
  year: 2012
  end-page: 412
  ident: CR10
  article-title: Genome-wide single-cell analysis of recombination activity and mutation rates in human sperm
  publication-title: Cell
  doi: 10.1016/j.cell.2012.06.030
– volume: 507
  start-page: 1944
  year: 2008
  end-page: 1951
  ident: CR35
  article-title: Aneuploid mosaicism in the developing and adult cerebellar cortex
  publication-title: J. Comp. Neurol.
  doi: 10.1002/cne.21648
– volume: 441
  start-page: 1087
  year: 2006
  end-page: 1093
  ident: CR25
  article-title: Generation of neuronal variability and complexity
  publication-title: Nature
  doi: 10.1038/nature04959
– volume: 3
  start-page: 1702
  year: 2007
  end-page: 1708
  ident: CR14
  article-title: Nanoliter reactors improve multiple displacement amplification of genomes from single cells
  publication-title: PLoS Genet.
  doi: 10.1371/journal.pgen.0030155
– volume: 491
  start-page: 56
  year: 2012
  end-page: 65
  ident: CR30
  article-title: An integrated map of genetic variation from 1,092 human genomes
  publication-title: Nature
  doi: 10.1038/nature11632
– volume: 19
  start-page: 455
  year: 2012
  end-page: 477
  ident: CR11
  article-title: SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing
  publication-title: J. Comput. Biol.
  doi: 10.1089/cmb.2012.0021
– volume: 105
  start-page: 15499
  year: 2008
  end-page: 15504
  ident: CR5
  article-title: A procedure for highly specific, sensitive, and unbiased whole-genome amplification
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0808028105
– volume: 29
  start-page: 915
  year: 2011
  end-page: 921
  ident: CR12
  article-title: Efficient assembly of single-cell bacterial genomes from short-read data sets
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1966
– volume: 23
  start-page: 878
  year: 2013
  end-page: 888
  ident: CR17
  article-title: Nearly finished genomes produced using gel microdroplet culturing reveal substantial intraspecies genomic diversity within the human microbiome
  publication-title: Genome Res.
  doi: 10.1101/gr.142208.112
– volume: 24
  start-page: 680
  year: 2006
  end-page: 686
  ident: CR1
  article-title: Sequencing genomes from single cells by polymerase cloning
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt1214
– volume: 31
  start-page: 533
  year: 2013
  end-page: 538
  ident: CR31
  article-title: Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.2579
– volume: 98
  start-page: 13361
  year: 2001
  ident: BFnbt2720_CR22
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.231487398
– volume: 2
  start-page: e558
  year: 2007
  ident: BFnbt2720_CR24
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0000558
– volume: 35
  start-page: W182
  year: 2007
  ident: BFnbt2720_CR39
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkm321
– volume: 5
  start-page: e10314
  year: 2010
  ident: BFnbt2720_CR16
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0010314
– volume: 441
  start-page: 1087
  year: 2006
  ident: BFnbt2720_CR25
  publication-title: Nature
  doi: 10.1038/nature04959
– volume: 39
  start-page: e19
  year: 2011
  ident: BFnbt2720_CR19
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkq1074
– volume: 23
  start-page: 826
  year: 2013
  ident: BFnbt2720_CR32
  publication-title: Genome Res.
  doi: 10.1101/gr.144600.112
– volume: 338
  start-page: 1622
  year: 2012
  ident: BFnbt2720_CR18
  publication-title: Science
  doi: 10.1126/science.1229164
– volume: 105
  start-page: 15499
  year: 2008
  ident: BFnbt2720_CR5
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0808028105
– volume: 23
  start-page: 878
  year: 2013
  ident: BFnbt2720_CR17
  publication-title: Genome Res.
  doi: 10.1101/gr.142208.112
– volume: 7
  start-page: 1024
  year: 2012
  ident: BFnbt2720_CR28
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2012.039
– volume: 25
  start-page: 2176
  year: 2005
  ident: BFnbt2720_CR21
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.4560-04.2005
– volume: 338
  start-page: 1627
  year: 2012
  ident: BFnbt2720_CR33
  publication-title: Science
  doi: 10.1126/science.1229112
– volume: 23
  start-page: 10454
  year: 2003
  ident: BFnbt2720_CR23
  publication-title: J. Neurosci.
  doi: 10.1523/JNEUROSCI.23-32-10454.2003
– volume: 24
  start-page: 680
  year: 2006
  ident: BFnbt2720_CR1
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt1214
– volume: 29
  start-page: 51
  year: 2011
  ident: BFnbt2720_CR3
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1739
– volume: 22
  start-page: 1139
  year: 2012
  ident: BFnbt2720_CR20
  publication-title: Genome Res.
  doi: 10.1101/gr.136242.111
– volume: 332
  start-page: 714
  year: 2011
  ident: BFnbt2720_CR7
  publication-title: Science
  doi: 10.1126/science.1203163
– volume: 31
  start-page: 533
  year: 2013
  ident: BFnbt2720_CR31
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.2579
– volume: 29
  start-page: 1072
  year: 2013
  ident: BFnbt2720_CR37
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btt086
– volume: 518
  start-page: 3981
  year: 2010
  ident: BFnbt2720_CR27
  publication-title: J. Comp. Neurol.
  doi: 10.1002/cne.22436
– volume: 17
  start-page: 377
  year: 2007
  ident: BFnbt2720_CR36
  publication-title: Genome Res.
  doi: 10.1101/gr.5969107
– volume: 309
  start-page: 1728
  year: 2005
  ident: BFnbt2720_CR29
  publication-title: Science
  doi: 10.1126/science.1117389
– volume: 471
  start-page: 58
  year: 2011
  ident: BFnbt2720_CR34
  publication-title: Nature
  doi: 10.1038/nature09871
– volume: 507
  start-page: 1944
  year: 2008
  ident: BFnbt2720_CR35
  publication-title: J. Comp. Neurol.
  doi: 10.1002/cne.21648
– volume: 150
  start-page: 402
  year: 2012
  ident: BFnbt2720_CR10
  publication-title: Cell
  doi: 10.1016/j.cell.2012.06.030
– volume: 19
  start-page: 455
  year: 2012
  ident: BFnbt2720_CR11
  publication-title: J. Comput. Biol.
  doi: 10.1089/cmb.2012.0021
– volume: 472
  start-page: 90
  year: 2011
  ident: BFnbt2720_CR8
  publication-title: Nature
  doi: 10.1038/nature09807
– volume: 29
  start-page: 915
  year: 2011
  ident: BFnbt2720_CR12
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1966
– volume: 3
  start-page: 1702
  year: 2007
  ident: BFnbt2720_CR14
  publication-title: PLoS Genet.
  doi: 10.1371/journal.pgen.0030155
– volume: 33
  start-page: 345
  year: 2010
  ident: BFnbt2720_CR26
  publication-title: Trends Neurosci.
  doi: 10.1016/j.tins.2010.04.001
– volume: 34
  start-page: e69
  year: 2006
  ident: BFnbt2720_CR15
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkl350
– volume: 148
  start-page: 873
  year: 2012
  ident: BFnbt2720_CR4
  publication-title: Cell
  doi: 10.1016/j.cell.2012.02.028
– volume: 102
  start-page: 17332
  year: 2005
  ident: BFnbt2720_CR13
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0508809102
– volume: 491
  start-page: 56
  year: 2012
  ident: BFnbt2720_CR30
  publication-title: Nature
  doi: 10.1038/nature11632
– volume: 4
  start-page: e6864
  year: 2009
  ident: BFnbt2720_CR2
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0006864
– volume: 104
  start-page: 11889
  year: 2007
  ident: BFnbt2720_CR6
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0704662104
– volume: 9
  start-page: 75
  year: 2008
  ident: BFnbt2720_CR38
  publication-title: BMC Genomics
  doi: 10.1186/1471-2164-9-75
– volume: 148
  start-page: 886
  year: 2012
  ident: BFnbt2720_CR9
  publication-title: Cell
  doi: 10.1016/j.cell.2012.02.025
– reference: 23707974 - Nat Biotechnol. 2013 Jun;31(6):533-8
– reference: 22817899 - Cell. 2012 Jul 20;150(2):402-12
– reference: 18261238 - BMC Genomics. 2008;9:75
– reference: 17620602 - Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11889-94
– reference: 16081699 - Science. 2005 Sep 9;309(5741):1728-32
– reference: 23493677 - Genome Res. 2013 May;23(5):878-88
– reference: 22506599 - J Comput Biol. 2012 May;19(5):455-77
– reference: 23128226 - Nature. 2012 Nov 1;491(7422):56-65
– reference: 20471112 - Trends Neurosci. 2010 Aug;33(8):345-54
– reference: 23422339 - Bioinformatics. 2013 Apr 15;29(8):1072-5
– reference: 18273885 - J Comp Neurol. 2008 Apr 20;507(6):1944-51
– reference: 22555242 - Nat Protoc. 2012 Jun;7(6):1024-41
– reference: 23258894 - Science. 2012 Dec 21;338(6114):1622-6
– reference: 16732271 - Nat Biotechnol. 2006 Jun;24(6):680-6
– reference: 23282328 - Genome Res. 2013 May;23(5):826-32
– reference: 17526522 - Nucleic Acids Res. 2007 Jul;35(Web Server issue):W182-5
– reference: 22385957 - Cell. 2012 Mar 2;148(5):873-85
– reference: 16810244 - Nature. 2006 Jun 29;441(7097):1087-93
– reference: 15745943 - J Neurosci. 2005 Mar 2;25(9):2176-80
– reference: 17255551 - Genome Res. 2007 Mar;17(3):377-86
– reference: 22385958 - Cell. 2012 Mar 2;148(5):886-95
– reference: 21170043 - Nat Biotechnol. 2011 Jan;29(1):51-7
– reference: 11698687 - Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13361-6
– reference: 17892324 - PLoS Genet. 2007 Sep;3(9):1702-8
– reference: 23258895 - Science. 2012 Dec 21;338(6114):1627-30
– reference: 22466172 - Genome Res. 2012 Jun;22(6):1139-43
– reference: 19724646 - PLoS One. 2009;4(9):e6864
– reference: 16286637 - Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17332-6
– reference: 21368824 - Nature. 2011 Mar 3;471(7336):58-62
– reference: 18832167 - Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15499-504
– reference: 20737596 - J Comp Neurol. 2010 Oct 1;518(19):3981-4000
– reference: 21551060 - Science. 2011 May 6;332(6030):714-7
– reference: 21071419 - Nucleic Acids Res. 2011 Mar;39(4):e19
– reference: 14614104 - J Neurosci. 2003 Nov 12;23(32):10454-62
– reference: 20428247 - PLoS One. 2010;5(4):e10314
– reference: 16707659 - Nucleic Acids Res. 2006;34(9):e69
– reference: 21399628 - Nature. 2011 Apr 7;472(7341):90-4
– reference: 17593959 - PLoS One. 2007;2(6):e558
– reference: 21926975 - Nat Biotechnol. 2011 Oct;29(10):915-21
SSID ssj0006466
Score 2.5305843
Snippet Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution....
Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations...
Arrays of nanoliter wells reduce bias in single-cell genome sequencing, allowing copy number changes in one cell to be detected at unprecedented resolution.
SourceID pubmedcentral
proquest
gale
pubmed
crossref
springer
SourceType Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 1126
SubjectTerms 631/61/514/2254
Agriculture
Bioinformatics
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cell Separation - instrumentation
Cells
Chromosome Mapping - instrumentation
Cloning
Cloning, Molecular - methods
Deoxyribonucleic acid
DNA
DNA - genetics
DNA polymerases
DNA sequencing
DNA-Directed DNA Polymerase - genetics
E coli
Equipment Design
Equipment Failure Analysis
Gene amplification
Genetic aspects
Genomics
High-Throughput Nucleotide Sequencing - instrumentation
High-Throughput Nucleotide Sequencing - methods
Life Sciences
Mammals
Methods
Microorganisms
Mutation
Nanotechnology - instrumentation
Nanotechnology - methods
Nucleotide sequencing
Physiological aspects
Reactors
Title Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells
URI https://link.springer.com/article/10.1038/nbt.2720
https://www.ncbi.nlm.nih.gov/pubmed/24213699
https://www.proquest.com/docview/1557612321
https://www.proquest.com/docview/1466375608
https://pubmed.ncbi.nlm.nih.gov/PMC3875318
Volume 31
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELdgEwgeEJSvQpnMBNpTWGLny09ooJWB1IEGQ32LHH9sk7KkkPah_z13rluaCigveWgu18hnn893v_yOkFcZ-PxQRyzQEA0EsbBpIGOpAxEbm9qIaemwOaPT9OQ8_jROxj7h1npY5dInOketG4U58kPY9zKkCmHR28mPALtGYXXVt9C4SXaRugxndTZeHbhgt3W1yijMEV6ZpEvyWZ4f1uX0DZYgO9vRplNe25U2EZMbZVO3Gw3vk3s-jKRHC7s_IDdM3SO3Fo0l5z1yd41msEduj3wB_SGxIwiWwcFVc4qk31VlKjppqjmmplpDVeWys1TWmiJ767WhHmuNvzaWYmahAjlTVS1FzPwFrWXduA-Z6TWC-zAZ2D4i58Pjb-9PAt9qIVB5mEwDwZUtVZaVkc0tM1ZnMo0MrFWRQASQMy3gJKMFtzwRKtIs1IqBYwzTnBlhueKPyU7d1OYpoaIMrYwjZUFzbK2QYISIyTS3YWKVzPrkYDnihfI85NgOoypcPZznBdimQNv0ycuV5GTBvfEHmddotMK37IRLi0mN9kLO2rY44hDNMp6G2RY55OSByCwGfftODqkxasTeLAQ-fv7eUfZ3oa2avp79hyYUWtN04IVsAyOlpP9yAsYbybs66gYdSXAVauvttf8ZLGd-4T1ZW_xed2CO1W18EtF5tWlmLR4fU55B7Jz3yZPFQlkZDCEHPBWiT7LOEloJIL959059del4zjmepSPQub9cbGuvtTEPnv373Z-TOwz7mDgc0oDsTH_OzAuIJqflnnMZcM2HH_bI7rvj0y9nvwB9HXbt
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6VIl4HBOEVCLBUVD2Z2rt-7QGhCggJbYoEbdWb2eyjILl2wIlQ_hS_kRk_QhwB4dJLDvFkYu3MzszOzH5DyPMIbL6rPeZoiAYcX9jQkb7UjvCNDa3HtCx7c0aH4eDYf38anG6Qn81dGGyrbGxiaah1rjBHvgt-L0KoEOa9mnxzcGoUVlebERqVWuyb-Q84shUvh29AvtuM9d8evR449VQBR8VuMHUEV3asomjs2dgyY3UkQ8-AWooAnF3MtICgXQtueSCUp5mrFQMb4IYxM8JyxYHvJXLZ5-DJ8WZ6_93C8odVbdRzY2znDMIG7JbHu9l4-gJLni33t-oElrzgaofmSpm29H79W-RmHbbSvUrPbpMNk3XIlWqQ5bxDbizBGnbI1VFdsL9D7AiCczCo6ZwiyHiampRO8nSOqbDCUJWW2WAqM00RLfbc0Lq3G7_NLcVMRgp0Jk0Lij36ZzSTWV5enKbn2EyIycfiLjm-ECHcI5tZnpkHhIqxa6XvKQucfWuFBCF4TIaxdQOrZNQlO82KJ6rGPcfxG2lS1t95nIBsEpRNlzxbUE4qrI8_0Gyj0JJ6RCh8FJhEKc7krCiSPQ7RM-OhG62hQwwg0B8f-G2VdAjFkWGvT0Uw_HDSYvZ3orWcPn38D05ItMRppyayOayUkvVNDVhvBAtrseu1KME0qbWPl_6n12h-UlvOIvm9z0Eci8f4S-wGzEw-K_C4GvIIYvW4S-5XG2UhMGxx4KEQXRK1ttCCAPHU20-yr19KXHWOZ3cPeG41m23ptVb04OG_3_0puTY4Gh0kB8PD_UfkOsMZKmUPVI9sTr_PzGOIZKfjJ6X5oOTzRdurX96KsbM
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6VVFRwQBBegQBLRdWTib3r1x4QKrRRQ0moSot6M5v1bkFy7YATofw1fh0zfoQ4AsKllxziycTamZ2ZnZn9hpAXAdh8O3aYFUM0YLnC-JZ0ZWwJVxvfOCyWRW_OcOQfnrnvzr3zDfKzvguDbZW1TSwMdZwpzJH3wO8FCBXCnJ6p2iKO9_uvJ98snCCFldZ6nEapIkd6_gOOb_mrwT7Ieoex_sHp20OrmjBgqdD2ppbgyoxVEIwdExqmTRxI39GgosIDxxeyWEAAHwtuuCeUEzM7Vgzsge2HTAvDFQe-18hmgKeiFtl8czA6Pln4Ab-slDp2iM2dnl9D3_Kwl46nL7EA2nCGqy5hySeu9muuFG0LX9i_TW5VQSzdK7XuDtnQaZtcL8daztvk5hLIYZtsDavy_V1ihhCqg3lN5hQhx5NEJ3SSJXNMjOWaqqTIDVOZxhSxYy81rTq98dvMUMxrJECnkySn2LF_QVOZZsU1anqJrYWYiszvkbMrEcN90kqzVD8kVIxtI11HGeDsGiMkCMFh0g-N7Rklgw7ZrVc8UhUKOg7jSKKiGs_DCGQToWw65PmCclIif_yBZgeFFlUDQ-Ejx5RKfiFneR7tcYilGfftYA0dIgJBXOgCv-2CDoE5UlTxkmDw4VOD2d-J1nL6ePIfnJBoidNuRWQyWCklq3sbsN4IHdZg121QgqFSax8v_U-31vyosqN59HvXgzgWj_GX2BuY6myW4-HV5wFE7mGHPCg3ykJg2PDAfSE6JGhsoQUBoqs3n6RfvxQo6xxP8g7w3K4329JrrejBo3-_-zOyBbYqej8YHT0mNxgOVCkaorqkNf0-008grJ2On1b2g5LPV22yfgFkELdF
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Massively+parallel+polymerase+cloning+and+genome+sequencing+of+single+cells+using+nanoliter+microwells&rft.jtitle=Nature+biotechnology&rft.au=Gole%2C+Jeff&rft.au=Gore%2C+Athurva&rft.au=Richards%2C+Andrew&rft.au=Chiu%2C+Yu-jui&rft.date=2013-12-01&rft.pub=Nature+Publishing+Group&rft.issn=1087-0156&rft.eissn=1546-1696&rft.volume=31&rft.issue=12&rft.spage=1126&rft_id=info:doi/10.1038%2Fnbt.2720&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=3415707621
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1087-0156&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1087-0156&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1087-0156&client=summon