Cathepsin E deficiency impairs autophagic proteolysis in macrophages

• Published in: PloS one Vol. 8; no. 12; p. e82415
• Main Authors: Tsukuba, Takayuki, Yanagawa, Michiyo, Kadowaki, Tomoko, Takii, Ryosuke, Okamoto, Yoshiko, Sakai, Eiko, Okamoto, Kuniaki, Yamamoto, Kenji
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.12.2013; Public Library of Science (PLoS)
• Subjects: Aberration; Abnormalities; Accumulation; Adenosine Triphosphate - genetics; Adenosine Triphosphate - metabolism; AKT protein; Animals; Antigens; Antioxidants; Apoptosis; Aspartic proteases; Aspartic proteinase; Autophagy; Biochemistry; Cathepsin E; Cathepsin E - genetics; Cathepsin E - metabolism; Cathepsins; Cell death; Compartments; Damage accumulation; Degradation; Dendritic cells; Depolarization; Extracellular signal-regulated kinase; Glutathione; Immunofluorescence; Immunoglobulins; Kinases; Laboratories; Lysosomes; Macrophages; Macrophages, Peritoneal - enzymology; Membrane potential; Membrane proteins; Membrane trafficking; Mice; Mice, Knockout; Microscopy; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Mitochondria; Mitochondria - genetics; Mitochondria - metabolism; Neurodegeneration; Organelles; Oxidative Stress; Oxygen; Oxygen consumption; Peroxiredoxin; Peroxiredoxin VI - genetics; Peroxiredoxin VI - metabolism; Phagocytosis; Pharmaceutical sciences; Proteinase; Proteins; Proteolysis; Rapamycin; Reactive oxygen species; Rodents; Signaling; Starvation; TOR protein; United States > US; Japan

Cathepsin E is an endosomal aspartic proteinase that is predominantly expressed in immune-related cells. Recently, we showed that macrophages derived from cathepsin E-deficient (CatE(-/-)) mice display accumulation of lysosomal membrane proteins and abnormal membrane trafficking. In this study, we d...