Compressive stress induces dephosphorylation of the myosin regulatory light chain via RhoA phosphorylation by the adenylyl cyclase/protein kinase A signaling pathway

• Published in: PloS one Vol. 10; no. 3; p. e0117937
• Main Authors: Takemoto, Kenji, Ishihara, Seiichiro, Mizutani, Takeomi, Kawabata, Kazushige, Haga, Hisashi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03.03.2015; Public Library of Science (PLoS)
• Subjects: Actin Cytoskeleton - metabolism; Activation; Adenylyl Cyclases - metabolism; Animals; Blood pressure; Cell Line; Chains; Compressing; Compression; Compressive properties; Compressive Strength - physiology; Cyclic AMP-Dependent Protein Kinases - metabolism; Deactivation; Deformation; Deformation mechanisms; Dephosphorylation; Enzyme inhibitors; Extracellular matrix; Fibroblasts; Guanosine triphosphate; Inactivation; Kinases; Life sciences; Light; Mice; Muscle proteins; Myosin; Myosin Light Chains - metabolism; Myosin-Light-Chain Kinase - antagonists & inhibitors; Myosin-Light-Chain Kinase - genetics; Myosin-Light-Chain Kinase - metabolism; Myosin-Light-Chain Phosphatase; Oxazoles - pharmacology; Phosphatase; Phosphorylation; Phosphorylation - drug effects; Protein kinase A; Proteins; Regulation; rho-Associated Kinases - metabolism; rhoA GTP-Binding Protein - metabolism; RhoA protein; RNA Interference; RNA, Small Interfering - metabolism; Shear stress; Signal transduction; Signal Transduction - drug effects; Signaling; Smooth muscle; Stress; Stresses; Stretching; United States > US; Japan

Mechanical stress that arises due to deformation of the extracellular matrix (ECM) either stretches or compresses cells. The cellular response to stretching has been actively studied. For example, stretching induces phosphorylation of the myosin regulatory light chain (MRLC) via the RhoA/RhoA-associ...