Bacillus subtilis as heterologous host for the secretory production of the non-ribosomal cyclodepsipeptide enniatin

• Published in: Applied microbiology and biotechnology Vol. 99; no. 2; pp. 681 - 691
• Main Authors: Zobel, Sophia, Kumpfmüller, Jana, Süssmuth, Roderich D, Schweder, Thomas
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.01.2015; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Amino acids; Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; bioactive properties; biochemical pathways; Biomedical and Life Sciences; bioprocess engineering; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; Cloning; Cloning, Molecular; Culture Media; Depsipeptides - biosynthesis; E coli; Enzymes; Fungi; Gene expression; Genetic aspects; genetic background; Genetic engineering; Gram-positive bacteria; Health aspects; hosts; Identification and classification; Industrial Microbiology; Life Sciences; ligases; metabolic engineering; Metabolic Engineering - methods; Metabolism; Metabolites; Microbial Genetics and Genomics; Microbiology; multigene family; Observations; Peptide Synthases - genetics; Peptide Synthases - metabolism; Peptides; Plasmids; Plasmids - genetics; Promoter Regions, Genetic; secondary metabolites; Streptococcus infections; Acetoin; Metabolic engineering; promoter; Cyclodepsipeptide; Iterative NRPS; Heterologous expression

The heterologous expression of genes or gene clusters in microbial hosts, followed by metabolic engineering of biosynthetic pathways, is key to access industrially and pharmaceutically relevant compounds in an economically affordable and sustainable manner. Therefore, platforms need to be developed,...