Rad51 recruitment and exclusion of non-homologous end joining during homologous recombination at a Tus/Ter mammalian replication fork barrier

• Published in: PLoS genetics Vol. 14; no. 7; p. e1007486
• Main Authors: Willis, Nicholas A., Panday, Arvind, Duffey, Erin E., Scully, Ralph
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.07.2018; Public Library of Science (PLoS)
• Subjects: Anemia; Animals; Base pairs; Biology and Life Sciences; Cancer; Cell Line; Chromatin; Competition; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA End-Joining Repair - genetics; DNA Polymerase theta; DNA Replication - genetics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; DNA-directed DNA polymerase; DNA-Directed DNA Polymerase - metabolism; Double-strand break repair; Gene conversion; Genetic recombination; Genetic research; Genomes; Hematology; Homologous recombination; Homologous Recombination - genetics; Immunoprecipitation; Intermediates; Ku Autoantigen - genetics; Ku Autoantigen - metabolism; Medical research; Medical schools; Medicine; Metabolism; Mice; Mice, Transgenic; Mouse Embryonic Stem Cells; Mutation; Nicking endonuclease; Non-homologous end joining; Oncology; Physical Sciences; Rad51 Recombinase - genetics; Rad51 Recombinase - metabolism; Replication; Replication fork; Research and Analysis Methods; Stem cells; Yeast; United States > US; Massachusetts

Classical non-homologous end joining (C-NHEJ) and homologous recombination (HR) compete to repair mammalian chromosomal double strand breaks (DSBs). However, C-NHEJ has no impact on HR induced by DNA nicking enzymes. In this case, the replication fork is thought to convert the DNA nick into a one-en...