Genome-wide quantification of 5′-phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics

• Published in: Nature protocols Vol. 11; no. 2; pp. 359 - 376
• Main Authors: Pelechano, Vicent, Wei, Wu, Steinmetz, Lars M
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.02.2016; Nature Publishing Group
• Subjects: 631/1647/514/1949; 631/337/1645/2020; 631/337/574; Analytical Chemistry; Biological Techniques; Codons; Computational Biology/Bioinformatics; Degradation; Degradation products; Depletion; Eukaryotic Cells - metabolism; Footprinting; Gene sequencing; Genetic research; Genetic translation; Genomes; Intermediates; Life Sciences; Messenger RNA; Methods; Microarrays; mRNA turnover; Next-generation sequencing; Oligonucleotides; Organic Chemistry; Properties; Protein Biosynthesis; protocol; Reverse transcription; Ribosomes; Ribosomes - metabolism; RNA sequencing; RNA Stability; rRNA; Saccharomyces cerevisiae; Translation

5PSeq is a method for studying ribosome dynamics based on co-translational mRNA decay. Genome-wide sequencing and quantification of 5′ phosphorylated mRNA degradation products allows the positions of the last translating ribosomes to be determined. Co-translational mRNA degradation is a widespread p...