Quantification of Arachidonic Acid and Its Metabolites in Rat Tissues by UHPLC-MS/MS: Application for the Identification of Potential Biomarkers of Benign Prostatic Hyperplasia

• Published in: PloS one Vol. 11; no. 11; p. e0166777
• Main Authors: Bian, Qiaoxia, Wang, Weihui, Wang, Nannan, Peng, Yan, Ma, Wen, Dai, Ronghua
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.11.2016; Public Library of Science (PLoS)
• Subjects: Acetonitrile; Acids; Ammonia; Analysis; Analytical chemistry; Animal tissues; Animals; Arachidonic acid; Arachidonic Acid - analysis; Arachidonic Acid - metabolism; Benign; Bioindicators; Biological markers; Biology and Life Sciences; Biomarkers; Biomarkers - analysis; Biomarkers - metabolism; Bladder; Calibration; Chromatography, High Pressure Liquid - methods; Column packings; Diagnosis; Dilution; Eicosanoids; Eicosanoids - analysis; Eicosanoids - metabolism; Enzymes; Formic acid; Genital diseases; Health aspects; Hyperplasia; Inflammation; Ionization; Kidney - metabolism; Kidneys; Lipids; Lipoxygenase; Liquid oxygen; Liver; Liver - metabolism; Male; Medicine and Health Sciences; Metabolism; Metabolites; Pharmaceuticals; Pharmacy; Prostaglandin E2; Prostaglandin endoperoxide synthase; Prostate; Prostate - metabolism; Prostate - pathology; Prostatic hyperplasia; Prostatic Hyperplasia - metabolism; Prostatic Hyperplasia - pathology; Rats, Sprague-Dawley; Reproducibility of Results; Rodents; Sensitivity and Specificity; Signal transduction; Tandem Mass Spectrometry - methods; Testosterone; Urinary Bladder - metabolism; Urology; China; United States > US

To evaluate the potential relationship between benign prostatic hyperplasia (BPH) and the arachidonic acid (AA) metabolome, a UHPLC-MS/MS method has been developed and validated for simultaneous determination of AA and its cyclooxygenase(COX) and lipoxygenase(LOX) pathway metabolites (15-HETE, 12-HE...