Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition

• Published in: PLoS biology Vol. 6; no. 10; p. e253
• Main Authors: Silva, Jose, Barrandon, Ornella, Nichols, Jennifer, Kawaguchi, Jitsutaro, Theunissen, Thorold W, Smith, Austin
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2008; Public Library of Science (PLoS)
• Subjects: Animals; Blotting, Northern; Brain - cytology; Brain - metabolism; Brain research; Cell Biology; Cells, Cultured; Cellular Reprogramming - genetics; Cellular Reprogramming - physiology; Councils; Developmental Biology; Efficiency; Epigenetics; Female; Flow Cytometry; Fluorescent Antibody Technique; In Situ Hybridization, Fluorescence; Kinases; Kruppel-Like Factor 4; Leukemia; Mice; Neurons - cytology; Neurons - metabolism; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction - genetics; Signal Transduction - physiology; Stem cells; Brain; Nuclear Reprogramming; Signal Transduction; Neurons; Cells, Cultured; In Situ Hybridization, Fluorescence; Reverse Transcriptase Polymerase Chain Reaction; Blotting, Northern; Animals; Flow Cytometry; Pluripotent Stem Cells; Fluorescent Antibody Technique; Female; Mice

Induced pluripotent stem (iPS) cells are generated from somatic cells by genetic manipulation. Reprogramming entails multiple transgene integrations and occurs apparently stochastically in rare cells over many days. Tissue stem cells may be subject to less-stringent epigenetic restrictions than othe...