Single Virus Genomics: A New Tool for Virus Discovery

• Published in: PloS one Vol. 6; no. 3; p. e17722
• Main Authors: Allen, Lisa Zeigler, Ishoey, Thomas, Novotny, Mark A., McLean, Jeffrey S., Lasken, Roger S., Williamson, Shannon J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.03.2011; Public Library of Science (PLoS)
• Subjects: Adaptations; Algorithms; Amplification; Bacteria; Bacteriophage lambda - genetics; Bacteriophage lambda - isolation & purification; Bacteriophage T4 - genetics; Bacteriophage T4 - isolation & purification; Biodiversity; Biological evolution; Biology; Cloning; Data processing; Data recovery; Deoxyribonucleic acid; DNA; DNA sequencing; E coli; Ecological effects; Ecology; Escherichia coli; Evolution; Flow Cytometry; Gene sequencing; Genetic aspects; Genetic Loci - genetics; Genome, Viral - genetics; Genomes; Genomics; Genomics - methods; Microorganisms; Microscopy; Microscopy, Confocal; Phages; Polymerase Chain Reaction; Principal components analysis; Reference Standards; Reproducibility of Results; Sequence Analysis, DNA; Venter, J Craig; Viruses; United States > US; California

Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of u...