Putative Porcine Embryonic Stem Cell Lines Derived from Aggregated Four-Celled Cloned Embryos Produced by Oocyte Bisection Cloning

• Published in: PLOS ONE Vol. 10; no. 2; p. e0118165
• Main Authors: Siriboon, Chawalit, Lin, Yu-Hsuan, Kere, Michel, Chen, Chun-Da, Chen, Lih-Ren, Chen, Chien-Hong, Tu, Ching-Fu, Lo, Neng-Wen, Ju, Jyh-Cherng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science (PLoS) 13.02.2015; Public Library of Science
• Subjects: Agricultural biotechnology; Alkaline phosphatase; Animal sciences; Animals; Apoptosis; Biomarkers; Biotechnology; Blastocyst; Blastocyst - cytology; Blastocysts; Blastomeres; Blastomeres - cytology; Bovidae; Cell culture; Cell Differentiation; Cell Line; Cell lines; Cell proliferation; Cell survival; Cells, Cultured; Cloning; Cloning, Organism; Colonies; Colonization; Comparative analysis; Culture Media; Efficiency; Embryo cells; Embryo Culture Techniques; Embryoid Bodies; Embryoid Bodies - cytology; Embryonic Stem Cells; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Embryos; Feeder Cells; Feeders; Female; Hogs; Medical research; Medicine; Oct-4 protein; Oocytes; Oocytes - cytology; Q; R; Research Article; Science; Stem cells; Suidae; Survival; Swine; Zoology; China; Taiwan

We attempted to isolate ES cell lines using inner cell masses from high-quality cloned porcine blastocysts. After being seeded onto feeders, embryos had better (P < 0.05) attachment, outgrowth formation and primary colonization in both 2× and 3× aggregated cloned embryos (62.8, 42.6 and 12.8% vs....