Genetic regulation of the ramA locus and its expression in clinical isolates of Klebsiella pneumoniae

• Published in: International journal of antimicrobial agents Vol. 38; no. 1; pp. 39 - 45
• Main Authors: Rosenblum, R., Khan, E., Gonzalez, G., Hasan, R., Schneiders, T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2011; Elsevier; Elsevier Science Publishers
• Subjects: acrA; Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; autoregulation; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Binding Sites; Biological and medical sciences; Citrobacter; Clinical isolates; complementary DNA; Drug Resistance, Bacterial - genetics; electrophoresis; Enterobacter; Gene Expression Regulation, Bacterial; Gene mapping; gene overexpression; genes; Genes, Bacterial; Humans; Infectious Disease; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Klebsiella pneumoniae - metabolism; loci; Medical sciences; Membrane Transport Proteins; Microbial Sensitivity Tests; Minocycline - analogs & derivatives; Minocycline - pharmacology; Molecular Sequence Data; Pharmacology. Drug treatments; promoter regions; Promoters; proteins; ramA; ramR; rapid amplification of cDNA ends; Regulatory sequences; Reverse Transcriptase Polymerase Chain Reaction; romA; Salmonella; Tigecycline; ramR; romA; acrA; Tigecycline; ramA; Klebsiella pneumoniae; Human; Glycylcycline derivatives; Antibiotic; Regulation(control); Bacteria; Genetics; Regulation; Antibacterial agent; Locus; Clinical isolate; Enterobacteriaceae

Tigecycline resistance has been attributed to ramA overexpression and subsequent acrA upregulation. The ramA locus, originally identified in Klebsiella pneumoniae, has homologues in Enterobacter and Salmonella spp. In this study, we identify in silico that the ramR binding site is also present in Ci...