Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells

• Published in: PLoS pathogens Vol. 15; no. 7; p. e1007907
• Main Authors: Mukhamedova, Nigora, Hoang, Anh, Dragoljevic, Dragana, Dubrovsky, Larisa, Pushkarsky, Tatiana, Low, Hann, Ditiatkovski, Michael, Fu, Ying, Ohkawa, Ryunosuke, Meikle, Peter J., Horvath, Anelia, Brichacek, Beda, Miller, Yury I., Murphy, Andrew, Bukrinsky, Michael, Sviridov, Dmitri
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.07.2019; Public Library of Science (PLoS)
• Subjects: ABCA1 protein; Actin; Activation; Alzheimer's disease; Alzheimers disease; Animals; Antiretroviral drugs; Apolipoproteins; Atherosclerosis; ATP Binding Cassette Transporter 1 - metabolism; ATP-binding protein; Biology and Life Sciences; Bystander Effect; Care and treatment; cdc42 GTP-Binding Protein - metabolism; Cdc42 protein; Cholesterol; Cholesterol - metabolism; Cytokines; Dementia; Diabetes; Drug therapy; Efflux; Exosomes; Exosomes - metabolism; Exosomes - virology; Extracellular signal-regulated kinase; Genetic aspects; Guanosine triphosphatases; Heart; HEK293 Cells; HIV; HIV infections; HIV Infections - metabolism; HIV Infections - virology; HIV patients; HIV-1; Human immunodeficiency virus; Humans; Immunology; Infection; Infections; Inflammasomes; Inflammation; Inflammation - metabolism; Inflammation - virology; Inflammatory response; Lipid metabolism; Lipid rafts; Lipids; Localization; Lymphocytes; Macrophages; Medicine; Medicine and health sciences; Membrane Microdomains - metabolism; Membrane Microdomains - virology; Metabolism; Mice; Mice, Inbred C57BL; Models, Biological; Monocytes; Monocytosis; Morbidity; Muscle proteins; nef Gene Products, Human Immunodeficiency Virus - genetics; nef Gene Products, Human Immunodeficiency Virus - metabolism; Nef protein; Pathogenesis; Phosphorylation; Polymerization; Proteins; Rafts; RAW 264.7 Cells; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Risk factors; Supervision; TLR4 protein; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Triggering Receptor Expressed on Myeloid Cells-1 - metabolism; Viral proteins; Australia; United States > US; Melbourne Victoria Australia
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1007907

HIV infection has a profound effect on "bystander" cells causing metabolic co-morbidities. This may be mediated by exosomes secreted by HIV-infected cells and containing viral factors. Here we show that exosomes containing HIV-1 protein Nef (exNef) are rapidly taken up by macrophages relea...