Genomic encoding of transcriptional burst kinetics

• Published in: Nature (London) Vol. 565; no. 7738; pp. 251 - 254
• Main Authors: Larsson, Anton J. M., Johnsson, Per, Hagemann-Jensen, Michael, Hartmanis, Leonard, Faridani, Omid R., Reinius, Björn, Segerstolpe, Åsa, Rivera, Chloe M., Ren, Bing, Sandberg, Rickard
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 2019; Nature Publishing Group
• Subjects: 13; 14; 14/32; 38; 38/91; 45; 631/208/200; 631/208/212/2019; 631/337/572/2102; Alleles; Animals; Biochemistry; Burst size; Bursting; Chemical synthesis; Confidence intervals; Enhancer Elements, Genetic - genetics; Enhancers; Fibroblasts - metabolism; Fluorescence; Gene expression; Gene sequencing; Genes; Genes - genetics; Genetic research; Genomes; Genomics; House mouse; Humanities and Social Sciences; Humans; Kinetics; Letter; Male; Methods; Mice; Mouse Embryonic Stem Cells - metabolism; multidisciplinary; Organ Specificity - genetics; Polymorphism, Genetic; Promoter Regions, Genetic - genetics; Promoters; Promoters (Genetics); Regulatory sequences; Ribonucleic acid; RNA; RNA polymerase; RNA sequencing; Science; Science (multidisciplinary); Sequence Analysis, RNA; Sequence Deletion; Single-Cell Analysis; Stem cells; Stochastic Processes; Synergistic effect; TATA Box - genetics; Transcription; Transcription (Genetics); Transcription, Genetic - genetics; Transcriptome - genetics; Trends; United States > US

Mammalian gene expression is inherently stochastic 1 , 2 , and results in discrete bursts of RNA molecules that are synthesized from each allele 3 – 7 . Although transcription is known to be regulated by promoters and enhancers, it is unclear how cis -regulatory sequences encode transcriptional burs...