Ebolavirus Is Internalized into Host Cells via Macropinocytosis in a Viral Glycoprotein-Dependent Manner

• Published in: PLoS pathogens Vol. 6; no. 9; p. e1001121
• Main Authors: Nanbo, Asuka, Imai, Masaki, Watanabe, Shinji, Noda, Takeshi, Takahashi, Kei, Neumann, Gabriele, Halfmann, Peter, Kawaoka, Yoshihiro
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.09.2010; Public Library of Science (PLoS)
• Subjects: Animals; Blotting, Western; Caveolae - metabolism; Caveolae - virology; Cell Biology; Cells, Cultured; Cercopithecus aethiops; Clathrin - metabolism; Ebola virus; Ebolavirus - physiology; Endocytosis - physiology; Gene expression; Genetic aspects; Hemorrhagic Fever, Ebola - metabolism; Hemorrhagic Fever, Ebola - virology; Humans; Infections; Infectious disease incubation period; Infectious Diseases/Viral Infections; Influenza; Kinases; Lasers; Microscopy, Fluorescence; Mortality; Physiological aspects; Pinocytosis; Pinocytosis - physiology; Plasma; Primates; Proteins; rab GTP-Binding Proteins - genetics; rab GTP-Binding Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Signal Transduction; Sorting Nexins - genetics; Sorting Nexins - metabolism; Studies; Vero Cells; Vesicular stomatitis virus; Vesiculovirus; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Virion - genetics; Virology/Host Invasion and Cell Entry; Virus Internalization; Virus Replication; Japan

Ebolavirus (EBOV) is an enveloped, single-stranded, negative-sense RNA virus that causes severe hemorrhagic fever with mortality rates of up to 90% in humans and nonhuman primates. Previous studies suggest roles for clathrin- or caveolae-mediated endocytosis in EBOV entry; however, ebolavirus virion...