Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation

• Published in: Nature (London) Vol. 467; no. 7315; pp. 591 - 595
• Main Authors: Mouquet, Hugo, Scheid, Johannes F., Zoller, Markus J., Krogsgaard, Michelle, Ott, Rene G., Shukair, Shetha, Artyomov, Maxim N., Pietzsch, John, Connors, Mark, Pereyra, Florencia, Walker, Bruce D., Ho, David D., Wilson, Patrick C., Seaman, Michael S., Eisen, Herman N., Chakraborty, Arup K., Hope, Thomas J., Ravetch, Jeffrey V., Wardemann, Hedda, Nussenzweig, Michel C.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.09.2010; Nature Publishing Group
• Subjects: 631/250/2152/2153/1291; 631/250/249/1570/1901; 631/326/596/2553; Antibodies; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibody Affinity - genetics; Antibody Affinity - immunology; Antigen-Antibody Reactions - genetics; Antigen-Antibody Reactions - immunology; Binding sites; Biochemistry; Biological and medical sciences; Cardiolipins - immunology; Cell Line, Tumor; Chemical properties; Competition; Cross Reactions - genetics; Cross Reactions - immunology; env Gene Products, Human Immunodeficiency Virus - immunology; Enzyme-Linked Immunosorbent Assay; Epitopes - chemistry; Epitopes - immunology; Epstein-Barr virus; Experiments; Fundamental and applied biological sciences. Psychology; Hepatitis C virus; HIV (Viruses); HIV Antibodies - genetics; HIV Antibodies - immunology; HIV Antigens - chemistry; HIV Antigens - immunology; HIV-1 - chemistry; HIV-1 - immunology; Human immunodeficiency virus; Humanities and Social Sciences; Humans; Immune response; Immunoglobulin Fab Fragments - genetics; Immunoglobulin Fab Fragments - immunology; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Heavy Chains - immunology; Immunology; letter; Microbiology; Miscellaneous; multidisciplinary; Mutation; Reactivity (Chemistry); Science; Science (multidisciplinary); Surface Plasmon Resonance; Viral antibodies; Virology; United States; Germany; Virus; Retroviridae; Antigen antibody reaction; Human immunodeficiency virus; Lentivirus

Antibodies hedge their bets Most antibodies are highly specific, binding with high affinity to a single foreign antigen. However, an analysis of human immunodeficiency virus (HIV) envelope glycoprotein-specific monoclonal antibodies from infected subjects provides evidence for a surprisingly high de...