Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus

• Published in: PLoS pathogens Vol. 14; no. 6; p. e1007124
• Main Authors: Kitamura, Kouichi, Que, Lusheng, Shimadu, Miyuki, Koura, Miki, Ishihara, Yuuki, Wakae, Kousho, Nakamura, Takashi, Watashi, Koichi, Wakita, Takaji, Muramatsu, Masamichi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.06.2018; Public Library of Science (PLoS)
• Subjects: Biology and life sciences; Cell culture; Chronic infection; Circular DNA; Cirrhosis; Cleavage; CRISPR; Deoxyribonucleic acid; DNA; DNA polymerase; DNA repair; DNA, Circular - genetics; DNA, Circular - metabolism; DNA, Viral - genetics; DNA, Viral - metabolism; DNA-directed DNA polymerase; Earth Sciences; Ecology and Environmental Sciences; Endonuclease; Enzyme Inhibitors - pharmacology; Etiology; FEN1 protein; Flap Endonucleases - antagonists & inhibitors; Flap Endonucleases - genetics; Flap Endonucleases - metabolism; Gene expression; Genetic aspects; Genetics; Genome editing; Genomes; Health aspects; Hep G2 Cells; Hepatitis; Hepatitis B; Hepatitis B - enzymology; Hepatitis B - genetics; Hepatitis B - virology; Hepatitis B virus; Hepatitis B virus - genetics; Hepatocellular carcinoma; Hepatocytes; Hepatocytes - drug effects; Hepatocytes - metabolism; Hepatocytes - virology; Humans; Immunoprecipitation; Infectious diseases; Liver; Liver cancer; Liver cirrhosis; Liver diseases; Medicine and health sciences; Mutation; Nucleocapsids; Proteins; Research and Analysis Methods; Saccharomyces cerevisiae; siRNA; Structure; Substrates; University graduates; Virion - enzymology; Virion - genetics; Virology; Virus Replication; Viruses; Japan

Hepatitis B virus (HBV) is one of the major etiological pathogens for liver cirrhosis and hepatocellular carcinoma. Chronic HBV infection is a key factor in these severe liver diseases. During infection, HBV forms a nuclear viral episome in the form of covalently closed circular DNA (cccDNA). Curren...