Wnt signaling regulates the lineage differentiation potential of mouse embryonic stem cells through Tcf3 down-regulation

• Published in: PLoS genetics Vol. 9; no. 5; p. e1003424
• Main Authors: Atlasi, Yaser, Noori, Rubina, Gaspar, Claudia, Franken, Patrick, Sacchetti, Andrea, Rafati, Haleh, Mahmoudi, Tokameh, Decraene, Charles, Calin, George A, Merrill, Bradley J, Fodde, Riccardo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.05.2013; Public Library of Science (PLoS)
• Subjects: Adenomatous Polyposis Coli Protein - genetics; Animals; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Biology; Cell Differentiation; Cell Lineage; Cloning; Colleges & universities; DNA Methylation; Down-Regulation; Embryonic stem cells; Embryonic Stem Cells - cytology; Embryonic Stem Cells - physiology; Gene expression; Gene Expression Regulation, Developmental; Genetic aspects; Genetic engineering; Kinases; Methylation; Mice; Mutation; Physiological aspects; Proteins; Stem cells; Transcription, Genetic; Wnt Signaling Pathway; United States

Canonical Wnt signaling plays a rate-limiting role in regulating self-renewal and differentiation in mouse embryonic stem cells (ESCs). We have previously shown that mutation in the Apc (adenomatous polyposis coli) tumor suppressor gene constitutively activates Wnt signaling in ESCs and inhibits the...