Synthesis of a probe for monitoring HSV1-tk reporter gene expression using chemical exchange saturation transfer MRI

• Published in: Nature protocols Vol. 8; no. 12; pp. 2380 - 2391
• Main Authors: Bar-Shir, Amnon, Liu, Guanshu, Greenberg, Marc M, Bulte, Jeff W M, Gilad, Assaf A
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2013; Nature Publishing Group
• Subjects: 631/1647/1888; 631/1647/245/1628; 631/1647/666; Analytical Chemistry; Animals; Biological Techniques; Biosensors; Computational Biology/Bioinformatics; Data acquisition; Data processing; Enzymes; Gene expression; Genes, Reporter; Genetic research; Herpes simplex virus 1; Herpes viruses; Herpesvirus 1, Human - enzymology; Hydrogenation; Identification and classification; Kinases; Life Sciences; Magnetic Resonance Imaging - methods; Male; Mice; Microarrays; Monitoring methods; Organic Chemistry; protocol; Rodentia - virology; Rodents; Thymidine - analogs & derivatives; Thymidine - analysis; Thymidine - chemical synthesis; Thymidine - chemistry; Thymidine Kinase - genetics; Thymidine Kinase - metabolism; Tomography; Viral Proteins - analysis; United States > US; Maryland; Baltimore Maryland

In experiments involving transgenic animals or animals treated with transgenic cells, it is important to have a method to monitor the expression of the relevant genes longitudinally and noninvasively. An MRI-based reporter gene enables monitoring of gene expression in the deep tissues of living subj...