Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

• Published in: BMC biotechnology Vol. 21; no. 1; p. 15
• Main Authors: Abdulrachman, Dede, Eurwilaichitr, Lily, Champreda, Verawat, Chantasingh, Duriya, Pootanakit, Kusol
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.02.2021; BioMed Central; BMC
• Subjects: 5-FOA; Aspergillus; Aspergillus - genetics; Aspergillus aculeatus; Bacterial Proteins - genetics; Biotechnology; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; CRISPR-Cas Systems; CRISPR/Cpf1; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Endonucleases - genetics; Enzymes; Feasibility studies; Filamentous fungi; FnCpf1; Francisella; Fungi; Gene disruption; Gene editing; Gene Editing - methods; Gene Expression Regulation, Fungal; gene targeting; Genes; Genetic aspects; Genetic engineering; genetic vectors; Genome editing; Genomes; Genomics; Homology; Localization; Methods; Microbial genetic engineering; Mutagenesis; Non-homologous end joining; Nuclease; Physiological aspects; Plasmids; pyrG; PyrG gene; Ribonucleic acid; RNA; RNA polymerase; RNA, Guide, CRISPR-Cas Systems; synthetic biology; Thailand; 5-FOA; CRISPR/Cpf1; Aspergillus; FnCpf1; Filamentous fungi; Gene editing; pyrG

CRISPR-Cas genome editing technologies have revolutionized biotechnological research particularly in functional genomics and synthetic biology. As an alternative to the most studied and well-developed CRISPR/Cas9, a new class 2 (type V) CRISPR-Cas system called Cpf1 has emerged as another versatile...