A top-down analysis of Xa- and Xi-territories reveals differences of higher order structure at ≥ 20 Mb genomic length scales

• Published in: Nucleus (Austin, Tex.) Vol. 2; no. 5; pp. 465 - 477
• Main Authors: Teller, Kathrin, Illner, Doris, Thamm, Stefanie, Casas-Delucchi, Corella S., Versteeg, Rogier, Indemans, Mireille, Cremer, Thomas, Cremer, Marion
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.09.2011
• Subjects: Binding; Biology; Bioscience; Calcium; Cancer; Cell; Cell Nucleus - ultrastructure; Cells, Cultured; chromatin; Chromosome Mapping; Chromosomes, Human, X - ultrastructure; Cycle; diploidy; DNA Probes - chemistry; Female; females; fibroblasts; fish; genes; genomics; histones; Humans; image analysis; Image Processing, Computer-Assisted; In Situ Hybridization, Fluorescence; Landes; Organogenesis; Proteins; RNA, Long Noncoding; RNA, Untranslated - analysis; RNA, Untranslated - ultrastructure; Sex Chromatin - ultrastructure; transcription (genetics)

The active and inactive X (Xa;Xi) territory with its seemingly highly compacted Barr body in nuclei of female mammalian cells provide a key example for studies of structure/function relationships in homologous chromosomes with different functional properties. Here we used about 300 human X-specific...