Evidence for Different Pathways during Horizontal Gene Transfer in Competent Bacillus subtilis Cells

• Published in: PLoS genetics Vol. 5; no. 9; p. e1000630
• Main Authors: Kidane, Dawit, Carrasco, Begoña, Manfredi, Candela, Rothmaier, Katharina, Ayora, Silvia, Tadesse, Serkalem, Alonso, Juan C., Graumann, Peter L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.09.2009; Public Library of Science (PLoS)
• Subjects: Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biochemistry/ and Repair; Cell Biology/Microbial Growth and Development; Deoxyribonucleic acid; Developmental Biology/Microbial Growth and Development; DNA; DNA - genetics; Gene Transfer, Horizontal; Genes; Genetic aspects; Genetic transformation; Genetics and Genomics/Microbial Evolution and Genomics; Microbiology; Molecular Biology/DNA Repair; Plasmids - genetics; Proteins; Spain

Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA. The integration of homologous donor DNA into the recipient chromosome requires RecA, while plasmid establishment, which is independent of R...