A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection

• Published in: PLoS biology Vol. 18; no. 12; p. e3001030
• Main Authors: Reijns, Martin A M, Thompson, Louise, Acosta, Juan Carlos, Black, Holly A, Sanchez-Luque, Francisco J, Diamond, Austin, Parry, David A, Daniels, Alison, O'Shea, Marie, Uggenti, Carolina, Sanchez, Maria C, O'Callaghan, Alan, McNab, Michelle L L, Adamowicz, Martyna, Friman, Elias T, Hurd, Toby, Jarman, Edward J, Chee, Frederic Li Mow, Rainger, Jacqueline K, Walker, Marion, Drake, Camilla, Longman, Dasa, Mordstein, Christine, Warlow, Sophie J, McKay, Stewart, Slater, Louise, Ansari, Morad, Tomlinson, Ian P M, Moore, David, Wilkinson, Nadine, Shepherd, Jill, Templeton, Kate, Johannessen, Ingolfur, Tait-Burkard, Christine, Haas, Jürgen G, Gilbert, Nick, Adams, Ian R, Jackson, Andrew P
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.12.2020; Public Library of Science (PLoS)
• Subjects: Biology and life sciences; Coronaviruses; COVID-19; COVID-19 - diagnosis; COVID-19 Testing - economics; COVID-19 Testing - methods; Disease control; Disease transmission; DNA probes; DNA-directed RNA polymerase; Epidemics; Fluorescein; Fluorescent indicators; Genes; Genomes; Humans; Innovations; Medicine and health sciences; Methods and Resources; Microbiological assay; Multiplex Polymerase Chain Reaction - economics; N gene; Nucleocapsids; Open reading frames; Pandemics; Polymerase chain reaction; Prices and rates; Probes; Protocol (computers); Public health; Quality control; Research and Analysis Methods; Respiratory diseases; Reverse Transcriptase Polymerase Chain Reaction - economics; Reverse transcription; Ribonucleic acid; RNA; RNA polymerase; RNA, Viral - analysis; RNA-directed RNA polymerase; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Target detection; Vaccines; Viral diseases; Viruses; United Kingdom; United States > US

With the ongoing COVID-19 (Coronavirus Disease 2019) pandemic, caused by the novel coronavirus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), there is a need for sensitive, specific, and affordable diagnostic tests to identify infected individuals, not all of whom are symptomatic. The...