Evaluation of nanoparticle tracking analysis for total virus particle determination

• Published in: Virology journal Vol. 9; no. 1; p. 265
• Main Authors: Kramberger, Petra, Ciringer, Mateja, Štrancar, Aleš, Peterka, Matjaž
• Format: Journal Article
• Language: English
• Published: London BioMed Central 12.11.2012; BioMed Central Ltd; BMC
• Subjects: Adenoviridae; Adenoviridae - isolation & purification; Adenovirus; Adenovirus 5; Adenoviruses; Atoms & subatomic particles; Biomedical and Life Sciences; Biomedicine; chromatography; CIM monolithic column; Comparative analysis; computer software; Diagnosis; Gene therapy; Health aspects; hemagglutination; Influenza; Influenza virus; Influenza viruses; latex; Latex particles; Methodology; Methods; Nanoparticles; NanoSight; NTA; Orthomyxoviridae; Orthomyxoviridae - isolation & purification; Polymerase chain reaction; Process controls; Reproducibility of Results; Software; Transmission electron microscopy; Vaccines; Viral Load - methods; virion; Virion - isolation & purification; Virology; Virus diseases; Viruses; Slovenia; Adenovirus 5; NTA; Latex particles; CIM monolithic column; Influenza; NanoSight
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-9-265

The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, adenovirus 5, and influenza virus. The inter-day variability was determined by measuring the same sample over several consecutive days and the method’s accuracy was demonstrated by using known concentrations of the subject particles. NTA analysis was also used to quantify chromatographic fractions of adenovirus and influenza virus after purification on a CIM monolithic column. NTA results were compared and evaluated against hemagglutination (HA) and end point dilution assay, determining total and infection virus particle number, respectively. The results demonstrated that nanoparticle tracking analysis is a method for fast estimation of virus concentration in different samples. In addition, it can provide a better insight into the sample status, regarding the level of virus aggregation.