Reverse Chemical Genetics: Comprehensive Fitness Profiling Reveals the Spectrum of Drug Target Interactions

• Published in: PLoS genetics Vol. 12; no. 9; p. e1006275
• Main Authors: Wong, Lai H, Sinha, Sunita, Bergeron, Julien R, Mellor, Joseph C, Giaever, Guri, Flaherty, Patrick, Nislow, Corey
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.09.2016; Public Library of Science (PLoS)
• Subjects: Alleles; Bayes Theorem; Biology and Life Sciences; Cancer; Cancer therapies; Colleges & universities; Dihydrofolate reductase; Drug interactions; Drug resistance; Drug Resistance, Neoplasm - genetics; Drug therapy; Folic Acid Antagonists - therapeutic use; Funding; Genetics; Humans; Library collections; Medicine and Health Sciences; Methotrexate - therapeutic use; Mutation; Neoplasms - drug therapy; Neoplasms - genetics; Observations; Pharmaceutical sciences; Pharmaceuticals; Research and Analysis Methods; Tetrahydrofolate Dehydrogenase - genetics; Tetrahydrofolate Dehydrogenase - metabolism; Massachusetts

The emergence and prevalence of drug resistance demands streamlined strategies to identify drug resistant variants in a fast, systematic and cost-effective way. Methods commonly used to understand and predict drug resistance rely on limited clinical studies from patients who are refractory to drugs...