Field-deployable multiplex detection method of SARS-CoV-2 and influenza virus using loop-mediated isothermal amplification and DNA chromatography

• Published in: PloS one Vol. 18; no. 5; p. e0285861
• Main Authors: Hayashida, Kyoko, Garcia, Alejandro, Moonga, Lavel Chinyama, Sugi, Tatsuki, Takuya, Kodera, Kawase, Mitsuo, Kodama, Fumihiro, Nagasaka, Atsushi, Ishiguro, Nobuhisa, Takada, Ayato, Kajihara, Masahiro, Nao, Naganori, Shingai, Masashi, Kida, Hiroshi, Suzuki, Yasuhiko, Hall, William W., Sawa, Hirofumi, Yamagishi, Junya
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.05.2023; Public Library of Science (PLoS)
• Subjects: Biology and life sciences; Chromatography; Coronaviruses; COVID-19; COVID-19 - diagnosis; Deoxyribonucleic acid; DNA; Engineering and Technology; Evaluation; Gene amplification; Humans; Influenza; Influenza A; Influenza B; Laboratories; Medicine and health sciences; Molecular Diagnostic Techniques - methods; Multiplexing; Nucleic Acid Amplification Techniques - methods; Orthomyxoviridae; Polymerase chain reaction; Research and analysis methods; Respiratory diseases; RNA, Viral - analysis; SARS-CoV-2 - genetics; Sensitivity analysis; Sensitivity and Specificity; Severe acute respiratory syndrome coronavirus 2; Target detection; Trehalose; Viral diseases; Viruses; Japan

A novel multiplex loop-mediated isothermal amplification (LAMP) method combined with DNA chromatography was developed for the simultaneous detection of three important respiratory disease-causing viruses: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus, and influenza...