A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species

• Published in: PloS one Vol. 10; no. 3; p. e0122004
• Main Authors: Ogawa, Hirohito, Fujikura, Daisuke, Ohnuma, Miyuki, Ohnishi, Naomi, Hang'ombe, Bernard M., Mimuro, Hitomi, Ezaki, Takayuki, Mweene, Aaron S., Higashi, Hideaki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.03.2015; Public Library of Science (PLoS)
• Subjects: Actin; Animal Diseases - diagnosis; Animal Diseases - microbiology; Animals; Anthrax; Anthrax - diagnosis; Anthrax - microbiology; Assaying; Bacillus; Bacillus anthracis; Bacillus anthracis - classification; Bacillus anthracis - genetics; Bacillus cereus; Bacillus cereus - classification; Bacillus cereus - genetics; Bacteria; Chromosomes, Bacterial; Deoxyribonucleic acid; Disease control; DNA; Epidemics; Genes; Genomes; Genotype & phenotype; Gram-Positive Bacterial Infections - diagnosis; Gram-Positive Bacterial Infections - microbiology; Health aspects; Humans; Identification methods; Infection; Infections; Infectious diseases; Intestine; Mammals; Multiplex Polymerase Chain Reaction; Multiplexing; Outbreaks; Phylogenetics; Plasmids; Plasmids - genetics; Polymerase chain reaction; Primers; Public health; Ribosomal DNA; Risk management; RNA; RNA, Bacterial; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sensitivity and Specificity; Strains (organisms); Veterinary colleges; Veterinary medicine; Virulence (Microbiology); Zoonoses; Europe; Japan; Zambia

Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has rec...