Impact of Colonoscopy Bowel Preparation on Intestinal Microbiota

• Published in: PloS one Vol. 8; no. 5; p. e62815
• Main Authors: O’Brien, Claire L., Allison, Gwen E., Grimpen, Florian, Pavli, Paul
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.05.2013; Public Library of Science (PLoS)
• Subjects: Aged; Antibiotics; Bacteria; Bacteroidetes - drug effects; Bacteroidetes - genetics; Bioinformatics; Biology; Bisacodyl - pharmacology; Cathartics - pharmacology; Cloning; Colon; Colonoscopy; Composition effects; Deoxyribonucleic acid; DNA; E coli; Fecal microflora; Feces; Feces - microbiology; Female; Gastroenterology; Gel electrophoresis; Gene sequencing; Genes; Hepatology; High-Throughput Nucleotide Sequencing; Hospitals; Host-bacteria relationships; Humans; Identification and classification; Inflammatory bowel disease; Intestinal microflora; Intestine; Intestines - microbiology; Male; Medicine; Methods; Microbiota; Microbiota (Symbiotic organisms); Microbiota - drug effects; Middle Aged; Molecular Typing; Next-generation sequencing; Patient outcomes; Phylogenetics; Phylogeny; Polyethylene glycol; Polyethylene Glycols - pharmacology; Primers; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, RNA; Australia; Canberra Australian Capital Territory Australia

The gut microbiota is important in maintaining human health, but numerous factors have the potential to alter its composition. Our aim was to examine the impact of a standard bowel preparation on the intestinal microbiota using two different techniques. Fifteen subjects undergoing colonoscopy consum...