Phenotype, function, and differentiation potential of human monocyte subsets

• Published in: PloS one Vol. 12; no. 4; p. e0176460
• Main Authors: Boyette, Lisa B., Macedo, Camila, Hadi, Kevin, Elinoff, Beth D., Walters, John T., Ramaswami, Bala, Chalasani, Geetha, Taboas, Juan M., Lakkis, Fadi G., Metes, Diana M.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.04.2017; Public Library of Science (PLoS)
• Subjects: Animal models; Antigen-presenting cells; Autoimmune diseases; Bioindicators; Biology and Life Sciences; Blood; Bone marrow; Cadmium; Cancer; CD14 antigen; CD16 antigen; CD34 antigen; CD4 antigen; Cell activation; Cell Differentiation - drug effects; Cell lineage; Cell proliferation; Cells, Cultured; Colony-stimulating factor; Cytotoxicity; Defensive behavior; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - immunology; Dendritic Cells - metabolism; Disease; Engineering; Gene expression; Genetic aspects; Genotype & phenotype; Granulocyte-macrophage colony-stimulating factor; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Heterogeneity; Homeostasis; Humans; Immunology; Immunophenotyping; Infections; Inflammation; Interleukin 3; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Ligands; Lymphocytes T; Macrophage colony stimulating factor; Macrophage Colony-Stimulating Factor - pharmacology; Macrophages; Macrophages - cytology; Macrophages - immunology; Macrophages - metabolism; Medicine; Medicine and Health Sciences; Mice; Microscopy, Fluorescence; Monocytes; Monocytes - cytology; Monocytes - metabolism; Nucleic acids; Organs; Pathogens; Phagocytosis - drug effects; Phenotype; Polarization; Research and Analysis Methods; Subpopulations; Surgery; T cell receptors; T cells; Tissues; Toll-Like Receptors - agonists; Toll-Like Receptors - metabolism; Transplantation; Tumor Necrosis Factor-alpha - metabolism; Urinary bladder; Viral infections; United States > US; Pittsburgh Pennsylvania

Human monocytes have been grouped into classical (CD14++CD16-), non-classical (CD14dimCD16++), and intermediate (CD14++CD16+) subsets. Documentation of normal function and variation in this complement of subtypes, particularly their differentiation potential to dendritic cells (DC) or macrophages, r...